Dataset Information

Multi-functions of McHB7 in Mesembryanthemum crystallinum responses to salt and drought stresses

ABSTRACT: Mesembryanthemum crystallinum (common ice plant) is one of the most important halophyte plants for plant stress biology research. In this study, we established an efficient Agrobacterium-mediated transformation method in ice plant and confirmed that ice plant can sustain gene overexpression for four weeks. Expression of a salt-induced transcription factor McHB7 (OE) reached the highest level at seven days after infiltration. Under salt and drought stresses, the growth of OE was better than wild type (WT), and the activities of redox enzymes and chlorophyll contents were higher in OE than the WT. Using proteomics, 475, 510 and 378 proteins were identified to be significantly changed in the OE lines under control, salt, and drought conditions, respectively. Most increased proteins were involved in various processes including Calvin cycle, citric acid cycle, glycolysis, and antioxidant pathways. Some were found to participate in ABA biosynthesis or response. Metabolomics revealed that many metabolites and phytohormones in OE were involved in plant growth and development. Also, ABA was increased in OE lines under control, salt, and drought conditions. Yeast one-hybrid analysis showed that McHB7 can bind to ERD and ABA-related motifs. And protein-protein interaction analysis discovered the candidate proteins that were responsive to stresses and hormones (e.g., ABA).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mesembryanthemum Crystallinum (common Ice Plant) (cryophytum Crystallinum)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Bowen Tan

LAB HEAD: Sixue Chen

PROVIDER: PXD033521 | Pride | 2024-05-21

REPOSITORIES: Pride

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Publications

Transcription Factor <i>Mc</i>HB7 Improves Ice Plant Drought Tolerance through ABA Signaling Pathway.

Zhang Xuemei X Cheng Zihan Z Fan Gaofeng G Zhu Dan D Tan Bowen B Jiang Tingbo T Chen Sixue S

International journal of molecular sciences 20240422 8

As global climate change continues, drought episodes have become increasingly frequent. Studying plant stress tolerance is urgently needed to ensure food security. The common ice plant is one of the model halophyte plants for plant stress biology research. This study aimed to investigate the functions of a newly discovered transcription factor, Homeobox 7 (HB7), from the ice plant in response to drought stress. An efficient <i>Agrobacterium</i>-mediated transformation method was established in t ...[more]

PMID: 38674154

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Project description:Understanding the genetic basis of plants’ response to environmental stresses such as drought and salinity is vital for improving the future crop productivity and for deciphering the evolutionary mechanisms of adaptation and speciation. Here, we screened for genes and functional groups that are potentially involved in drought tolerance in tomato by comparing genome-wide transcriptome profiles of drought-sensitive S. lycopersicum and drought-tolerant S. pimpinellifolium populations under control and water deficit conditions. We also compared the transcriptome profiles from this study and a previous salt treatment study to investigate expression similarities and differences in gene expression patterns between water and salt stress responses, which are physiologically and biochemically similar. Stress-induced genes such as dehydration responsive element binding (DREB) protein, ABA-response element binding factor (AREB)-like protein, heat shock proteins, and chaperones were commonly up-regulated in S. lycopersicum and S. pimpinellifolium. Genes such as WRKY transcription factors and 1-aminocyclopropane-1-carboxylate (ACC) synthase exhibited striking differences in both the baseline expression under the control condition as well as expression changes in response to water deficit, suggesting that the two species have accumulated heritable differences in gene expression patterns. At the genome scale, there was a tendency that down-regulated genes in S. lycopersicum are more neutral or even up-regulated in S. pimpinellifolium, suggesting that S. pimpinellifolium may be able to maintain cellular activities during prolonged droughts. In comparison of water and salt stress responses, known stress-induced genes such as DREB protein, AREB-like protein, and nine-cis-epoxycarotenoid dioxygenase (NCED) were commonly up-regulated in response to these stresses. However, we also found fundamental differences between these stress responses in terms of genome-wide expression patterns, partly attributable to the difference in how these stresses were applied during the experiments. We compared Affymetrix microarray transcriptome profiles of root tissues from three natural populations each of S. lycopersicum and S. pimpinellifolium (3-4 individuals in each population as biological replicates under control (well-watered) and water deficit treatments.

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Dataset Information

Multi-functions of McHB7 in Mesembryanthemum crystallinum responses to salt and drought stresses

Dataset's files

Publications

Transcription Factor <i>Mc</i>HB7 Improves Ice Plant Drought Tolerance through ABA Signaling Pathway.

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