Proteomics

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Overexpression of McHB7 Transcription Factor from Mesembryanthemum crystallinum Improves Plant Salt Tolerance


ABSTRACT: Mesembryanthemum crystallinum (common ice plant) is one of the facultative halophyte plants, and it serves as a model for investigating the molecular mechanisms underlying its salt stress response and tolerance. Here we cloned one of homeobox transcription factor (TF) gene McHB7 from ice plant, which has 60% similarity with the Arabidopsis AtHB7. Overexpression of McHB7 in Arabidopsis (OE) showed that the plants had significantly elevated relative water content (RWC), chlorophyll content, superoxide dismutase (SOD) and peroxidase (POD) activities after salt stress treatment. Proteomics analysis identified 145 to be significantly changed in abundance, and 66 were exclusively increased in the OE plants compared to wild type (WT). After salt treatment, 979 and 959 metabolites were significantly increased and decreased in OE plants compared to the WT, respectively. The results demonstrated McHB7 can improve photosynthesis and increase the leaf chlorophyll content, and affect TCA cycle by regulating metabolites (e.g., pyruvate) and proteins (e.g., citrate synthase). Also, McHB7 modulates the expression of stress-related proteins (e.g., superoxide dismutase, dehydroascorbate reductase and pyrroline-5-carboxylate synthase B) to scavenge reactive oxygen species and enhance plant salt tolerance.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Bowen Tan  

LAB HEAD: Sixue Chen

PROVIDER: PXD033886 | Pride | 2022-08-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
XM_OC1.msf Msf
XM_OC1.raw Raw
XM_OC2.raw Raw
XM_OC3.raw Raw
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Publications

Overexpression of <i>McHB7</i> Transcription Factor from <i>Mesembryanthemum crystallinum</i> Improves Plant Salt Tolerance.

Zhang Xuemei X   Tan Bowen B   Cheng Zihan Z   Zhu Dan D   Jiang Tingbo T   Chen Sixue S  

International journal of molecular sciences 20220717 14


<i>Mesembryanthemum crystallinum</i> (common ice plant) is one of the facultative halophyte plants, and it serves as a model for investigating the molecular mechanisms underlying its salt stress response and tolerance. Here we cloned one of the homeobox transcription factor (TF) genes, <i>McHB7</i>, from the ice plant, which has 60% similarity with the Arabidopsis <i>AtHB7</i>. Overexpression of the <i>McHB7</i> in Arabidopsis (OE) showed that the plants had significantly elevated relative water  ...[more]

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