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Complexome profiling of mitochondria to study NDUFAF1 mediated complex I assembly in Yarrowia lipolytica

ABSTRACT: The aerobic yeast Yarrowia lipolytica is an established model organism to study mitochondrial protein complexes. Here we performed a complexome analysis of intact mitochondria to study the function of NDUFAF1 in the assembly of respiratory complex I. In the ndufaf1 deletion strain the PD module of the membrane arm accumulated but mature complex I was virtually absent.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Yarrowia Lipolytica (candida Lipolytica)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig

LAB HEAD: Volker Zickermann

PROVIDER: PXD033522 | Pride | 2022-12-21

REPOSITORIES: Pride

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			Action	DRS
	Data_analysis.xlsx	Xlsx
	Native_Gel.jpg	Other
	P20_107_Jonathan_KO_01.raw	Raw
	P20_107_Jonathan_KO_02.raw	Raw
	P20_107_Jonathan_KO_03.raw	Raw

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