Proteomics

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Phosphoproteome of hippocampus after focal cerebral ischemia 1.5 h and reperfusion 24 h in mice


ABSTRACT: We obtained the profiles of neuronal phosphoproteome after cerebral ischemia and reperfusion by isolating mice hippocampus. Hippocampus combined from either nine sham or nine focal cerebral ischemia 1.5 h and reperfusion 24 h (IR) mice were lysed, digested, labeled with different TMT tags, then pooled and analyzed by LC/LC-MS/MS. In total, we quantified 7,865 phosphopeptides,179 phosphorylation sites of 129 proteins were upregulated and 843 phosphorylation sites of 494 proteins were downregulated in hippocampus during cerebral ischemia 2 h compare with sham operation.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Wei Jiang  

LAB HEAD: Haijun Tu

PROVIDER: PXD033851 | Pride | 2024-02-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Z8063TPST_1.raw Raw
Z8063TPST_2.raw Raw
Z8063TPST_3.raw Raw
Z8063TPST_4.raw Raw
Z8063TPST_5.raw Raw
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