Phosphoproteome of hippocampus after focal cerebral ischemia 1.5 h and reperfusion 24 h in mice
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ABSTRACT: We obtained the profiles of neuronal phosphoproteome after cerebral ischemia and reperfusion by isolating mice hippocampus. Hippocampus combined from either nine sham or nine focal cerebral ischemia 1.5 h and reperfusion 24 h (IR) mice were lysed, digested, labeled with different TMT tags, then pooled and analyzed by LC/LC-MS/MS. In total, we quantified 7,865 phosphopeptides,179 phosphorylation sites of 129 proteins were upregulated and 843 phosphorylation sites of 494 proteins were downregulated in hippocampus during cerebral ischemia 2 h compare with sham operation.
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: Wei Jiang
LAB HEAD: Haijun Tu
PROVIDER: PXD033851 | Pride | 2024-02-15
REPOSITORIES: Pride
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