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The eWAT proteomics of wild type mice and Mdm2 adipocyte-specific knock-in mice fed high-fat diet

ABSTRACT: The epididymal adipose tissue (eWAT) samples from wild type mice fed high-fat diet for 12 weeks (H_WT_E) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (H_KI_E) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectrometry-based proteomics techniques, etc. The proteomics of mixed eWAT samples were performed in Jingjie PTM BioLab (Hangzhou) Co. Ltd (www.ptm-biolab.com.cn). The difference was determined by 1.5-fold-change criterion, FDR < 0.01.

INSTRUMENT(S): LTQ Orbitrap, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Whole Body

SUBMITTER: Xiaojun Liu

LAB HEAD: Xiaojun Liu

PROVIDER: PXD034074 | Pride | 2022-08-11

REPOSITORIES: Pride

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			Action	DRS
	F9527LQB1_H_KI_E.raw	Raw
	F9527LQB1_H_WT_E.raw	Raw
	Search_result.zip	Other
	checksum.txt	Txt
	mqpar.xml	Xml

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Publications

Murine double minute 2 aggravates adipose tissue dysfunction through ubiquitin-mediated six-transmembrane epithelial antigen of prostate 4 degradation.

Zhao Wei W Xu Qiang Q Yang Jiahui J Xie Xianghong X Li Chunmei C Zhang Weihong W Chen Enhui E Guo Yanfang Y Gao Mingyue M Shi Jie J Zhang Huabing H Yao Hong H Li Meixia M Yan Li L Fang Fude F Wu Wenming W Liu Xiaojun X

iScience 20220607 7

Healthy adipose tissue is crucial to maintain normal energy homeostasis. Little is known about the role of murine double minute 2 (MDM2), an E3 ubiquitin ligase and has been highlighted in oncopathology, in adipose tissue. Our results indicated that MDM2 expression was associated with nutritional status. <i>Mdm2</i> adipocyte-specific knock-in (<i>Mdm2</i>-AKI) mice exhibited exacerbated weight gain, insulin resistance, and decreased energy expenditure. Meanwhile, chronic high-fat diet (HFD) exp ...[more]

PMID: 35747386

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Project description:The serum samples from wild type mice fed high-fat diet for 12 weeks (WT_Serum) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (KI_Serum) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectrometry-based proteomics techniques in Jingjie PTM BioLab (Hangzhou) Co. Ltd (www.ptm-biolab.com.cn). The difference was determined by 1.5-fold-change criterion, FDR < 0.01.

2022-08-11 | PXD034069 | Pride

The eWAT ubiquitinome of wild type mice and Mdm2 adipocyte-specific knock-in mice fed high-fat diet

Project description:The epididymal adipose tissue (eWAT) samples from wild type mice fed high-fat diet for 12 weeks (H_WT_E) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (H_KI_E) were mixed separately, and subjected to ubiquitinome study by Label-free quantification and ubiquitination modification enrichment techniques, etc. The proteomics of mixed eWAT samples were performed in Jingjie PTM BioLab (Hangzhou) Co. Ltd (www.ptm-biolab.com.cn). The difference was determined by 1.5-fold-change criterion, FDR < 0.01.

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high fat diet feed mice liver sample proteomics

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Project description:The popularity of high fat foods in modern society has been associated with epidemic of various metabolic diseases characterized by insulin resistance, the pathology of which involves complex interactions between multiple tissues such as liver, skeletal muscle and white adipose tissue (WAT). To uncover the mechanism by which excessive fat impairs insulin sensitivity, we conducted a multi- tissue study by using TMT-based quantitative proteomics. 3-week-old ICR mice were fed with high fat diet (HFD) for 19 weeks to induce insulin resistance. Liver, skeletal muscle and epididymal fat were collected for proteomics screening. Additionally, PRM was used for validating adipose differential proteins. By comparing tissue-specific protein profiles of HFD mice, multi-tissue regulation of glucose and lipid homeostasis and corresponding underlying mechanisms was systematically investigated and characterized. NC: normal birth weight + chow diet; NH: normal birth weight + high fat diet; LC: low birth weight + chow diet; LH: low birth weight + high fat diet.

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