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Quantitative proteomics profiling of spermatozoa and seminal plasma reveals proteins associated with semen quality in Bos indicus bulls

ABSTRACT: Primary aim of the analysis was to identify proteomic differences in the semen samples of Bos indicus that accounts for high or low ERR. This was achieved through tandem mass-tags based quantitative proteomics approach coupled to LC-MS/MS methods. An Orbitrap Fusion Tribrid mass spectrometer (Thermo Fischer Scientific, Bremen, Germany) connected to the Easy- nLC-1200 nanoflow liquid chromatography system (Thermo Scientific) was used for data acquisition.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Testis, Sperm

SUBMITTER: Keshava Prasad T. S.

LAB HEAD: T. S. Keshava Prasad

PROVIDER: PXD034096 | Pride | 2024-07-15

REPOSITORIES: pride

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			Action	DRS
	NDRI_Semen_Proteomics_28042022.pdResult	Other
	NDRI_TMT_F1_R1.raw	Raw
	NDRI_TMT_F1_R2.raw	Raw
	NDRI_TMT_F1_R3.raw	Raw
	NDRI_TMT_F2_R1.raw	Raw

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Publications

Quantitative proteomics profiling of spermatozoa and seminal plasma reveals proteins associated with semen quality in Bos indicus bulls.

Ashwitha A A Ramesha Kerekoppa P KP Ramesh Poornima P Kootimole Chinmaya Narayana CN Devadasan M Joel MJ Ammankallu Shruthi S Jeyakumar Sakthivel S Kumaresan Arumugam A Veerappa Vedamurthy Gowdar VG Das D N DN Prasad Thottethodi Subrahmanya Keshava TSK

Journal of proteomics 20221216

Cattle breeding approaches are an evolving field of research in veterinary science. Certain factors such as Ejaculate Rejection Rate (ERR) pose a limitation to such approaches. In this regard, we sought to investigate the spermatozoa and seminal plasma proteome of Hallikar bulls with low (n = 3) and high (n = 3) ERR. Through the Tandem mass spectrometry approach, we identified a total of 2409 proteins, in which 828 proteins were common in both the semen components, whereas 375 and 378 proteins w ...[more]

PMID: 36535621

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