Proteomics

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Exploring molecular dynamics indicators associated with reproductive performance of Bos indicus cattle in plasma samples through data-independent acquisition mass spectrometry


ABSTRACT: Aim of the study was to profile the blood plasma proteome of healthy Bos indicus cattle and use it as a baseline against which to compare different conditions in Bos indicus cattle, such as disease, reproduction associated proteins and pregnancy specific proteins etc. Data-independent acquisition (DIA) bases quantitative proteomics approach coupled to LC-MS/MS methods were used to achieve this aim. An Orbitrap Fusion Tribrid mass spectrometer (Thermo Fischer Scientific, Bremen, Germany) connected to the Easy- nLC-1200 nanoflow liquid chromatography system (Thermo Scientific) was used for data acquisition.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Plasma

SUBMITTER: Keshava Prasad T. S.  

LAB HEAD: T. S. Keshava Prasad

PROVIDER: PXD034103 | Pride | 2024-07-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
DIA_120Min_NDRI_A3.raw Raw
DIA_120Min_NDRI_A4.raw Raw
DIA_120Min_NDRI_A5.raw Raw
DIA_120Min_NDRI_A6.raw Raw
DIA_120Min_NDRI_D4.raw Raw
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Publications

Exploring molecular dynamic indicators associated with reproductive performance of Bos indicus cattle in blood plasma samples through data-independent acquisition mass spectrometry.

Devadasan M Joel MJ   Ramesha Kerekoppa P KP   Ramesh Poornima P   Kootimole Chinmaya Narayana CN   Jeyakumar Sakthivel S   Ashwitha A A   Ammankallu Shruthi S   Rai Akhila Balakrishna AB   Kumaresan Arumugam A   Vedamurthy Veerappa G VG   Raju Rajesh R   Das D N DN   Kataktalware Mukund A MA   Prasad Thottethodi Subrahmanya Keshava TSK  

Journal of proteomics 20230613


Improving reproductive performance of cattle is of paramount importance for sustainable dairy farming. Poor reproduction performance (RP) hinders the genetic improvement of important Bos indicus cattle breeds. It is well known that incorporation of molecular information along with conventional breeding method is far better than use of conventional method alone for the genetic improvement of reproductive performance traits in cattle. Therefore, the present study sought to investigate the plasma p  ...[more]

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