Dataset Information

Impact of neonatal lead exposure on memory: A study highlighting the association between hippocampal synaptic proteome and cognitive decline in rats

ABSTRACT: Lead (Pb2+) is a heavy metal ubiquitously present in the environment, with unknown essential functions to sustain life. The detrimental effects of Pb2+ on health are known since ancient times, which is also well supported by scientific literature. Incidentally, Pb2+ toxicity is more severe in children than in adults. Various studies highlight the drastic effects of Pb2+ exposure on memory and cognition. However, the exact signalling pathways affected by Pb2+ are still unclear. In the present study, we exposed rat pups to Pb2+ acetate through maternal lactation from day P0 till weaning. The impact of early Pb2+ exposure to spatial cognition was tested using partially baited radial arm maze task three months after weaning. Furthermore, to study the effect of Pb2+ exposure on SUMOylation, the synaptosomal fractions from the hippocampus were purified, and mass spectrometry-based proteomic analysis was performed. Results from behavioral tests revealed that Pb2+ exposure early in life caused severe impairments in spatial cognition with increased reference memory errors and a reduced number of correct choices in PBRAM test. The proteomic analysis revealed differentially altered proteins in Pb2+ exposed rats compared to controls, including SUMO target proteins. Thus, our findings suggest that SUMOylation is a vital protein modification that plays an essential role in memory consolidation.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Bhupesh Mehta

LAB HEAD: Dr. Bhupesh Mehta

PROVIDER: PXD034212 | Pride | 2023-03-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Rat_Synaptosomes_TMT_10_PLEX_triplicates_PROTEOMICS__091521.msf	Msf
	Rat_Synaptosomes_TMT_10_PLEX_triplicates_PROTEOMICS__091521.pdResult	Other
	Synaptosome_F10_R1.raw	Raw
	Synaptosome_F10_R2.raw	Raw
	Synaptosome_F10_R3.raw	Raw

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Publications

A proteomic study to unveil lead toxicity-induced memory impairments invoked by synaptic dysregulation.

Mohanraj Nivedha N Joshi Neha S NS Poulose Roshni R Patil Rahul R RR Santhoshkumar Rashmi R Kumar Anubhav A Waghmare Girish P GP Saha Amit Kumar AK Haider Syeda Zehra SZ Markandeya Yogananda S YS Dey Gourav G Rao Laxmi T LT Govindaraj Periyasamy P Mehta Bhupesh B

Toxicology reports 20220707

Lead (Pb<sup>2+</sup>), a ubiquitously present heavy metal toxin, has various detrimental effects on memory and cognition. However, the molecular processes affected by Pb<sup>2+</sup> causing structural and functional anomalies are still unclear. To explore this, we employed behavioral and proteomic approaches using rat pups exposed to lead acetate through maternal lactation from postnatal day 0 (P0) until weaning. Behavioral results from three-month-old rats clearly emphasized the early life Pb ...[more]

PMID: 36518382

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