Proteomics

Dataset Information

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Changes to SUMO-modified proteome and total cellular proteome during EBV ractivation in AGS gastric carcinoma cells

ABSTRACT: To monitor changes to cellular and viral SUMO-modified proteins during EBV reactivation. SUMO1 and SUMO2 conjugates were studied using KGG mutant forms of SUMO and GG-K specific antibody enrichment of SUMO modified peptides. Time-points of 12h and 24h post-reactivation were monitored. Total cellular proteome was also analysed to study changes to the total protein levels of proteins under the same conditions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Gastric Adenocarcinoma

SUBMITTER: Mike Tatham  

LAB HEAD: Ronald T. Hay

PROVIDER: PXD034631 | Pride | 2023-06-19

REPOSITORIES: Pride

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Dataset's files

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20190815_MT_RTH_LF_GGK_90m_top4_300ms_sample01_LysC.raw Raw
20190815_MT_RTH_LF_GGK_90m_top4_300ms_sample02_LysC.raw Raw
20190815_MT_RTH_LF_GGK_90m_top4_300ms_sample03_LysC.raw Raw
20190815_MT_RTH_LF_GGK_90m_top4_300ms_sample04_LysC.raw Raw
20190815_MT_RTH_LF_GGK_90m_top4_300ms_sample05_LysC.raw Raw
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Publications

Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1.

De La Cruz-Herrera Carlos F CF   Tatham Michael H MH   Siddiqi Umama Z UZ   Shire Kathy K   Marcon Edyta E   Greenblatt Jack F JF   Hay Ronald T RT   Frappier Lori L  

PLoS pathogens 20230706 7

SUMO modifications regulate the function of many proteins and are important in controlling herpesvirus infections. We performed a site-specific proteomic analysis of SUMO1- and SUMO2-modified proteins in Epstein-Barr virus (EBV) latent and lytic infection to identify proteins that change in SUMO modification status in response to EBV reactivation. Major changes were identified in all three components of the TRIM24/TRIM28/TRIM33 complex, with TRIM24 being rapidly degraded and TRIM33 being phospho  ...[more]

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