Proteomics

Dataset Information

0

Proteomic analysis of a targeted functional genomic screen in human skeletal muscle organoids

ABSTRACT: To begin to validate potential causal regulators of muscle function, we targeted genes containing novel skeletal muscle pQTLs and molecular/phenotypic associations, and performed a functional genomic screen in human skeletal muscle organoids (PMID: 30527761). We focused on proteins with negative associations to lean mass, grip strength or other metabolic associations, and generated a total of 27 individual rAAV6:shRNAs. Organoids were grown around contraction posts to monitor contractile force production during electrical stimulation, and transduced following differentiation and maturation to limit effects on the myogenic program (Figure 3A). Electrical stimulation was performed to induce either a tetanic contraction for assessment of maximum force production or stimulated with sustained lower frequency for assessment of fatigue. Following the protocol, organoids were analysed by proteomics which quantified 17/27 targets with 13 targets significantly reduced in abundance by rAAV6:shRNA.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skeletal Muscle Cell Line

SUBMITTER: Benjamin Parker  

LAB HEAD: Benjamin Parker

PROVIDER: PXD034913 | Pride | 2023-01-08

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
210608_Exploris3_BP-2388_AAV_SkMorganoid_Exp1_A1.raw Raw
210608_Exploris3_BP-2388_AAV_SkMorganoid_Exp1_A2.raw Raw
210608_Exploris3_BP-2388_AAV_SkMorganoid_Exp1_A3.raw Raw
210608_Exploris3_BP-2388_AAV_SkMorganoid_Exp1_A4.raw Raw
210608_Exploris3_BP-2388_AAV_SkMorganoid_Exp1_A5.raw Raw
Items per page:
1 - 5 of 145
altmetric image

Publications

Improving muscle function has great potential to improve the quality of life. To identify novel regulators of skeletal muscle metabolism and function, we performed a proteomic analysis of gastrocnemius muscle from 73 genetically distinct inbred mouse strains, and integrated the data with previously acquired genomics and >300 molecular/phenotypic traits via quantitative trait loci mapping and correlation network analysis. These data identified thousands of associations between protein abundance a  ...[more]

Similar Datasets

Proteomics AP-MS of ALPK3 in cardiomyocytes
2023-01-19 | PXD035734 | Pride
Proteomics Proteomic analysis of the cardiac muscle from the HMDP
2023-06-01 | PXD036398 | Pride
Proteomics Proteomic and phosphoproteomic of C18ORF25 KO skeletal muscle following electrical contraction
2022-07-18 | PXD031101 | Pride
Proteomics Solvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
2022-08-04 | PXD028736 | Pride
Proteomics Phosphoproteomics of cardiac organoids derived from ALPK3 knockout hPSCs
2023-01-19 | PXD035535 | Pride
Proteomics Long-term nutritional regulation of liver proteome
2021-12-13 | PXD028928 | Pride
Proteomics Circ101093 regulated proteins in Lung carcinoma (LUAD) cells.
2022-05-20 | PXD025491 | Pride
Proteomics Proteomic analysis of skeletal muscle from the Hybryid Mouse Diversity Panel
2023-01-08 | PXD032729 | Pride
Proteomics Cryo-EM visualizes substrate channeling and E2 multisite phosphorylation configuring ubiquitin ligation by GID/CTLH E3
2023-12-18 | PXD043646 | Pride
Proteomics Proteomic analysis of UFC1 knockdown in mouse skeletal muscle
2023-01-08 | PXD035170 | Pride