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Actinomycin D treated cytotoxic T lymphocytes

ABSTRACT: Effector cytotoxic T lymphocytes (CTLs) are critical for ridding the body of infected or cancerous cells. Recognition of an antigenic ligand by the CTL’s T cell receptor (TCR) triggers a signalling cascade that ultimately results in the targeted release of cytolytic granules and/or secretion of cytokines and chemokines to alert and recruit additional immune cells. These signalling cascades are capable of initiating transcription, translation, and cytoskeletal rearrangements. While previous work has demonstrated how translation and intracellular reorganisation contribute to CTL effector responses, the role of transcription is less well studied. To address this, we examined the impact of blocking transcription on the CTL proteome during TCR stimulation. These data demonstrated a strong transcriptional requirement for expression of cytokines and chemokines but not cytolytic molecules. Together with functional studies, these data reveal differential molecular control of the cell-cell communication and cytolytic functions of effector CTLs. CTLs exhibit complete and persistent priming for cytolytic activity prior to target cell encounter, but they require de novo transcription to recruit additional immune cells that amplify the response.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell, Cell Culture

SUBMITTER: Julia Marchingo

LAB HEAD: Gillian M. Griffiths

PROVIDER: PXD034920 | Pride | 2023-10-16

REPOSITORIES: Pride

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			Action	DRS
	20200519_124106_JM-DIA.sne	Other
	20200830_183359_JM-DIA_Report_peptides.setup.txt.txt	Txt
	20200830_183359_JM-DIA_Report_peptides.xls.xls	Xls
	20200830_184021_JM_DIA_Report_protein.xls.xlsx	Xlsx
	20200830_184031_JM-DIA_Report_protein.setup.txt.txt	Txt

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