Dataset Information

Fish coelomic fluid proteomic analysis by LC-MSMS

ABSTRACT: The aim of this study was to complete and fully characterize the proteome of rainbow trout coelomic fluid (CF). Furthermore, we intended to identify the proteins responsible for the extension of egg viability in salmonids coelomic fluid. For this, the proteomic composition of different salmonids CF and non-salmonids CF were compared to identify proteins specifically present in salmonids CF. Those proteins could be responsible for the egg conservation property of the salmonids CF. In parallel, rainbow trout CF has been fractionated using HPLC coupled to gel filtration or ion exchange columns. We have then tested the egg conservation properties of the different fractions obtained. Proteins present in the fractions allowing egg quality preservation were identified. Identifying the CF components involved in egg viability would have important applied outputs with various biotechnological applications for aquaculture. It could help understanding egg quality preservation mechanisms, and consequently improving egg storage condition.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Cyprinus Carpio (common Carp) Acipenser Baerii Salmo Trutta Acipenser Gueldenstaedtii Carassius Auratus Oncorhynchus Mykiss (rainbow Trout) (salmo Gairdneri) Sander Lucioperca Salmo Salar (atlantic Salmon)

TISSUE(S): Ovarian Fluid

SUBMITTER: Aurelie Gueho

LAB HEAD: Julien Bobe

PROVIDER: PXD034989 | Pride | 2024-05-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	296_170_Slot1-16_1_420.d.zip	Other
	296_170_Slot1-16_1_420_DA5.1.201_1.mgf	Mgf
	296_171_Slot1-17_1_422.d.zip	Other
	296_171_Slot1-17_1_422_DA5.1.201_1.mgf	Mgf
	296_172_Slot1-10_1_432.d.zip	Other

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Publications

Evolutionarily conserved ovarian fluid proteins are responsible for extending egg viability in salmonid fish.

Gueho Aurélie A Żarski Daniel D Rime Hélène H Guével Blandine B Com Emmanuelle E Lavigne Régis R Nguyen Thaovi T Montfort Jérôme J Pineau Charles C Bobe Julien J

Scientific reports 20240426 1

In contrast to most fishes, salmonids exhibit the unique ability to hold their eggs for several days after ovulation without significant loss of viability. During this period, eggs are held in the body cavity in a biological fluid, the coelomic fluid (CF) that is responsible for preserving egg viability. To identify CF proteins responsible for preserving egg viability, a proteomic comparison was performed using 3 salmonid species and 3 non-salmonid species to identify salmonid-specific highly ab ...[more]

PMID: 38671194

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