Proteomics

Dataset Information

0

Human UFSP1 is an active protease that regulates UFM1 maturation and UFMylation


ABSTRACT: An essential first step in the posttranslational modification of proteins with UFM1, UFMylation, is the proteolytic cleavage of pro-UFM1 to expose a C-terminal glycine. Of the two UFM1-specific proteases (UFSPs) identified in humans, only UFSP2 is reported to be active since the annotated sequence of UFSP1 lacks critical catalytic residues. Nonetheless, efficient UFM1 maturation occurs in cells lacking UFSP2 suggesting the presence of another active protease. We hereby identify UFSP1 translated from a non-canonical start site to be this protease. Cells lacking both UFSPs show complete loss of UFMylation resulting from an absence of mature UFM1. While UFSP2, but not UFSP1, removes UFM1 from the ribosomal subunit RPL26, UFSP1 acts earlier in the pathway to mature UFM1 and cleave a potential auto-inhibitory modification on UFC1, thereby controlling activation of UFMylation. In summary, our studies reveal distinctions in substrate specificity and localization-dependent functions for the two proteases in regulating UFMylation. The present dataset includes Data Independent Acquisition (DIA) quantitative proteomics analysis of knockout cell lines reported in our study. Our analysis of the processed data is presented in Figure-6.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Frederic Lamoliatte  

LAB HEAD: Yogesh Kulathu

PROVIDER: PXD035142 | Pride | 2022-08-22

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
DIANN_output.zip Other
DMillrine20210526_DKO1.raw Raw
DMillrine20210526_DKO2.raw Raw
DMillrine20210526_DKO3.raw Raw
DMillrine20210526_UFSP1-1.raw Raw
Items per page:
1 - 5 of 14
altmetric image

Publications


An essential first step in the post-translational modification of proteins with UFM1, UFMylation, is the proteolytic cleavage of pro-UFM1 to expose a C-terminal glycine. Of the two UFM1-specific proteases (UFSPs) identified in humans, only UFSP2 is reported to be active, since the annotated sequence of UFSP1 lacks critical catalytic residues. Nonetheless, efficient UFM1 maturation occurs in cells lacking UFSP2, suggesting the presence of another active protease. We herein identify UFSP1 translat  ...[more]

Similar Datasets

2018-12-11 | GSE123539 | GEO
2024-04-22 | GSE264185 | GEO
2024-03-22 | PXD046990 | Pride
2022-11-22 | PXD038157 | Pride
2024-03-22 | PXD046991 | Pride
2024-01-29 | GSE253809 | GEO
2023-01-20 | GSE222104 | GEO
2023-07-20 | PXD041336 | Pride
2022-03-09 | GSE186287 | GEO
2024-09-16 | PXD048568 | Pride