Proteomics

Dataset Information

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Proteomic analysis of the extracellular vesicles of the filamentous fungus Botrytis cinerea.

ABSTRACT: Botrytis cinerea is the causing agent of grey mould on hundreds of plants cultivated in fields, tunnels and greenhouses worldwide. Considering the central role of secretion in the necrotrophic interaction between the fungus and its host plants, non conventional secre-tion was explored through the search and characterisation of extracellular vesicles. In or-der to reveal the protein composition of these vesicles, a proteomic analysis was chosen.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Botryotinia Fuckeliana (noble Rot Fungus) (botrytis Cinerea)

TISSUE(S): Extracellular Vesicle

SUBMITTER: Dupuy Jean-William  

LAB HEAD: Christophe Bruel

PROVIDER: PXD035766 | Pride | 2023-05-02

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
ENSEMBL_Botrytis_cinerea_ASM83294v1_Release47.fasta Fasta
Link_between_files.xlsx Xlsx
cb191003_01.msf Msf
cb191003_01.pdResult Other
cb191003_01.raw Raw
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Publications

Fungal secretomes are known to contain a multitude of components involved in nutrition, cell growth or biotic interactions. Recently, extra-cellular vesicles have been identified in a few fungal species. Here, we used a multidisciplinary approach to identify and characterize extracellular vesicles produced by the plant necrotroph <i>Botrytis cinerea</i>. Transmission electron microscopy of infectious hyphae and hyphae grown in vitro revealed extracellular vesicles of various sizes and densities.  ...[more]

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