Proteomics

Dataset Information

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Comparative proteomic analysis of the exoproteome of a wild type and cps1 mutant strains of the filamentous fungus Botrytis cinerea.


ABSTRACT: Botrytis cinerea is the causing agent of grey mould on hundreds of plants cultivated in fields, tunnels and greenhouses worldwide, including economically important crops. Considering the central role of the fungal cell wall in the interaction between fungi and plants, the role of a conserved putative polysaccharide synthase in the physiology, development and virulence of B. cinerea was explored. To this aim, the BcCps1 gene was deleted and the mutant strain was characterized. In order to reveal the impact of the mutation on the fungal secretion, the exo-proteome of the wild type and mutant strains were prepared for comparative analysis of their contents.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Botryotinia Fuckeliana (strain B05.10) (noble Rot Fungus) (botrytis Cinerea)

SUBMITTER: Dupuy Jean-William  

LAB HEAD: Christophe Bruel

PROVIDER: PXD033590 | Pride | 2022-10-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ENSEMBL_Botrytis_cinerea_ASM83294v1_Release53.fasta Fasta
Link_between_files.xlsx Xlsx
cb210930_01.msf Msf
cb210930_01.pdResult Other
cb210930_01.raw Raw
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Publications

Evidencing New Roles for the Glycosyl-Transferase Cps1 in the Phytopathogenic Fungus <i>Botrytis cinerea</i>.

Blandenet Matthieu M   Gonçalves Isabelle R IR   Rascle Christine C   Dupuy Jean-William JW   Gillet François-Xavier FX   Poussereau Nathalie N   Choquer Mathias M   Bruel Christophe C  

Journal of fungi (Basel, Switzerland) 20220824 9


The fungal cell wall occupies a central place in the interaction between fungi and their environment. This study focuses on the role of the putative polysaccharide synthase Cps1 in the physiology, development and virulence of the grey mold-causing agent <i>Botrytis cinerea</i>. Deletion of the <i>Bccps1</i> gene does not affect the germination of the conidia (asexual spores) or the early mycelial development, but it perturbs hyphal expansion after 24 h, revealing a two-phase hyphal development t  ...[more]

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