Proteomics

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Proteomic analysis of several non pathogenic mutants from Botrytis cinerea.

ABSTRACT: The grey mould fungus Botrytis cinerea is a typical necrotrophic fungus that can infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to find new important genes involved in the fungal virulence, a mutant library was generated by random insertional mutagenesis, using Agrobacterium tumefaciens mediated transformation. Ten mutants exhibiting total loss of virulence towards different host plants were considered. Their molecular characterization revealed a single T-DNA insertion in unique and different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably proteases and hemicellulases deficiencies, were observed and validated by biochemical tests. They were also a hallmark of the remaining six non pathogenic strains, suggesting the importance of these secreted proteins in the infectious process.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Botryotinia Fuckeliana (noble Rot Fungus) (botrytis Cinerea)

SUBMITTER: Dupuy Jean-William  

LAB HEAD: Nathalie Poussereau

PROVIDER: PXD013359 | Pride | 2021-11-03

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Ensembl_Botrytis_cinerea_ASM15095v2_31.fasta Fasta
Linkbetweenfiles.xlsx Xlsx
vg170502_01.msf Msf
vg170502_01.raw Raw
vg170502_02.msf Msf
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Publications

The gray mold fungus <i>Botrytis cinerea</i> is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated by random insertional mutagenesis using <i>Agrobacterium tumefaciens-</i>mediated transformation (ATMT). Twelve mutants exhibiting total loss of virulence toward different host plants were chosen for detailed analyses. Their molecul  ...[more]

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