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TESMIN interactome characterization

ABSTRACT: We performed immunoprecipitation followed by mass spectrometry (IP-MS) and proximity-dependent biotin identification (BioID) to capture TESMIN interactome.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Seiya Oura

LAB HEAD: Masahito Ikawa

PROVIDER: PXD035916 | Pride | 2023-03-11

REPOSITORIES: Pride

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	11983.d.zip	Other
	11985.d.zip	Other
	11987.d.zip	Other
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Publications

Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins.

Oura Seiya S Ninomiya Akinori A Sugihara Fuminori F Matzuk Martin M MM Ikawa Masahito M

Scientific reports 20221223 1

Characterization of protein-protein interactions (PPI) is a key to understanding the functions of proteins of interest. Recently developed proximity-dependent biotin identification (BioID) has been actively investigated as an alternative PPI mapping method because of its usefulness in uncovering transient PPI. Here, as an example of proximity labeling proteomics application in the testis, we generated two transgenic mouse lines expressing two biotin ligases (BioID2 or TurboID) fused with TESMIN, ...[more]

PMID: 36564444

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