Dataset Information

Uncovering the protein alterations in anther tissues of three contrasting rice types in response to short-term and long-term heat stress conditions

ABSTRACT: Heat stress drastically affects anther tissues resulting in poor plant fertility, necessitating an urgent need to determine the key proteins associated in the response of anthers to heat stress. Employing quantitative proteome analysis, we identified several cultivar-specific protein alterations in anthers of Moroberekan (Japonica, heat sensitive), IR64 (Indica, moderately heat tolerant), and Nagina 22 (Aus, heat tolerant) rice types following short-term (ST_HS; one cycle of 42°C, 4 hours before anthesis) and long-term (LT_HS; 6 cycles of 38°C, 6 hours before anthesis) heat treatments. The protein numbers were higher in Nagina 22 than IR 64 and Moroberekan anthers under unstressed and heat stress. The proteins upregulated in long-term heat stress in Nagina 22 were enriched in biological processes related to unfolded protein binding and carboxylic acid metabolism, including amino acid metabolism. In short-term heat stress, Nagina 22 anthers were enriched in proteins associated with vitamin E biosynthesis while proteins downregulated were related to ribosomal proteins. The expression of Hsp20, DnaJ, and Hsp101 was predominantly observed in Nagina 22. Overall, the heat response in Nagina 22 was associated with its capacity for adequate metabolic control and cellular homeostasis which may be critical for its higher reproductive thermotolerance

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Anther

SUBMITTER: Palak Chaturvedi

LAB HEAD: Palak Chaturvedi

PROVIDER: PXD035952 | Pride | 2023-02-17

REPOSITORIES: Pride

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Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes.

Kumar Ritesh R Ghatak Arindam A Goyal Isha I Sarkar Neelam K NK Weckwerth Wolfram W Grover Anil A Chaturvedi Palak P

Frontiers in plant science 20230113

Heat stress drastically affects anther tissues resulting in poor plant fertility, necessitating an urgent need to determine the key proteome regulation associated with mature anther in response to heat stress. We identified several genotype - specific protein alterations in rice anthers of Moroberekan (Japonica, heat sensitive), IR64 (Indica, moderately heat tolerant), and Nagina22 (Aus, heat tolerant) in the short-term (ST_HS; one cycle of 42°C, 4 hours before anthesis) and long-term (LT_HS; 6 ...[more]

PMID: 36756226

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Project description:Effects of heat priming applied to the first generation on tolerance of the successive generation to post-anthesis high temperature stress were investigated. Compared with the progeny of non-heat primed plants (NH), the progeny of heat-primed plants (PH) presented higher grain yield, leaf photosynthesis and activities of antioxidant enzymes and lower cell membrane damage under high temperature stress. In the transcriptome profile, 1430 probes showed obvious difference in expression between PH and NH. These genes were those of signal transduction, transcription, energy, defense, and protein destination and storage, respectively. Pot experiments (25 cm in diameter and 22 cm in depth) were conducted at the Experimental Station of Nanjing Agricultural University, Nanjing, China (32Ë30Ê¹N and 118Ë42Ê¹E). During the first generation, wheat plants were divided into two groups: one group were not primed (N) while the other were heat-primed (P) at pre-anthesis (at a day/night temperature of 32/28ËC for two days at the seven-leaf stage and the nine-leaf stage) and post-anthesis (34/30ËC for seven days at the 10th day after anthesis). At maturity, seeds from both groups were harvested. Seedlings (the progeny generation) from each group seed were further divided two sub-groups: one was subjected to high temperature stress at a day/night temperature of 34/30ËC, while the other was set at 26/22ËC for six days from the 10th day after anthesis (DAA). During both priming and high-temperature stress, plants under different treatments were moved into sepatarate growth chambers at preset temperatures. Thereafter, four treatments were established: NC, progeny of non-primed plants without post-anthesis high temperature stress; NH, progeny of non-primed plants with post-anthesis high temperature stress; PC, progeny of primed plants without post-anthesis high temperature stress; and PH, progeny of primed plants with post-anthesis high temperature stress.

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