Dataset Information

VAMP2/3-dependent membrane proteome in oligodendrocytes

ABSTRACT: Which proteins in oligodendrocytes require VAMP2/3-mediated exocytosis for membrane trafficking? We isolated primary oligodendrocytes from transgenic mice expressing oligodendrocyte-targeted botulinum toxin and littermate controls and used surface biotinylation to determine which proteins were differentially trafficked to the cell surface.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell, Oligodendrocyte

SUBMITTER: Mable Lam

LAB HEAD: J. Bradley Zuchero

PROVIDER: PXD036174 | Pride | 2022-10-14

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20210603_MLam_Zuchero_11506_ctrl.raw_20210607_Byonic.mgf	Mgf
	20210603_MLam_Zuchero_11506_iBot.raw_20210607_Byonic.mgf	Mgf
	20210811_MLam_Zuchero_11697_ctrl_01.raw	Raw
	20210811_MLam_Zuchero_11697_ctrl_01.raw_20210816_Byonic.byparms	Raw
	20210811_MLam_Zuchero_11697_ctrl_01.raw_20210816_Byonic.mgf	Mgf

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Publications

CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes.

Lam Mable M Takeo Koji K Almeida Rafael G RG Cooper Madeline H MH Wu Kathryn K Iyer Manasi M Kantarci Husniye H Zuchero J Bradley JB

Nature communications 20220923 1

Myelin is required for rapid nerve signaling and is emerging as a key driver of CNS plasticity and disease. How myelin is built and remodeled remains a fundamental question of neurobiology. Central to myelination is the ability of oligodendrocytes to add vast amounts of new cell membrane, expanding their surface areas by many thousand-fold. However, how oligodendrocytes add new membrane to build or remodel myelin is not fully understood. Here, we show that CNS myelin membrane addition requires e ...[more]

PMID: 36151203

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