Dataset Information

Turning spherical cells into kinking helices in wall-less bacteria

ABSTRACT: In bacteria, cell shape is determined and maintained through a complex interplay between the peptidoglycan cell wall and cytoplasmic filaments made of polymerized MreB filaments. Spiroplasma species, of the Mollicutes class, challenge this general understanding because they are characterized by a helical cell shape and kink-based motility without a cell wall. This specificity is thought to rely on 5 MreB isoformsand a specific fibril protein. In this study, comninations of these 5 MreBs and the fibril from S. citri were expressed in another Mollicute, Mycoplamsa capricolum. The abundance of these proteins were compared in S.citri at different stages of growth, as well as in Mcap transformants.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mycoplasma Capricolum Subsp. Capricolum Spiroplasma Citri Bacteria

SUBMITTER: Julie Hardouin

LAB HEAD: Julie Hardouin

PROVIDER: PXD036290 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	10_MreB1-5Fib_cl32-1-6P_02.msf	Msf
	10_MreB1-5Fib_cl32-1-6P_02.raw	Raw
	10_MreB1-5Fib_cl32-1-6P_03.msf	Msf
	10_MreB1-5Fib_cl32-1-6P_03.raw	Raw
	10_MreB1-5Fib_cl32-1-6P_04.msf	Msf

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Publications

Cytoskeletal components can turn wall-less spherical bacteria into kinking helices.

Lartigue Carole C Lambert Bastien B Rideau Fabien F Dahan Yorick Y Decossas Marion M Hillion Mélanie M Douliez Jean-Paul JP Hardouin Julie J Lambert Olivier O Blanchard Alain A Béven Laure L

Nature communications 20221114 1

Bacterial cell shape is generally determined through an interplay between the peptidoglycan cell wall and cytoplasmic filaments made of polymerized MreB. Indeed, some bacteria (e.g., Mycoplasma) that lack both a cell wall and mreB genes consist of non-motile cells that are spherical or pleomorphic. However, other members of the same class Mollicutes (e.g., Spiroplasma, also lacking a cell wall) display a helical cell shape and kink-based motility, which is thought to rely on the presence of five ...[more]

PMID: 36376306

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