Dataset Information

Cell-based characterization of caspase-3 cleavage sites by LATE and HYATNE

ABSTRACT: Proteins' N-termini contain information about their biochemical properties and functions, and they can undergo co- or post-translational modifications, as well as be processed by proteases. To expand the coverage of the N-terminome, we have developed LATE (LysN Amino Terminal Enrichment), a method that uses selective chemical derivatization of α-amines to isolate the N-terminal peptides. We applied LATE in combination with other N-terminomic method to study caspase-3 mediated proteolysis both in vitro and during apoptosis in cells. This enable us to identify many unreported caspase-3 cleavages, including some that cannot be identified by other methods. Furthermore, we find direct evidence that some of the caspase-3 generated neo-N-termini can be further modified by Nt-acetylation. This provides a global picture of the caspase-3 degradome and uncovers previously unrecognised functional crosstalk between post-translational Nt-acetylation and caspase proteolysis pathways.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Oded Kleifeld

LAB HEAD: Oded Kleifeld

PROVIDER: PXD036648 | Pride | 2023-06-27

REPOSITORIES: Pride

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Publications

In-Depth Characterization of Apoptosis N-Terminome Reveals a Link Between Caspase-3 Cleavage and Posttranslational N-Terminal Acetylation.

Hanna Rawad R Rozenberg Andrey A Saied Layla L Ben-Yosef Daniel D Lavy Tali T Kleifeld Oded O

Molecular & cellular proteomics : MCP 20230524 7

The N termini of proteins contain information about their biochemical properties and functions. These N termini can be processed by proteases and can undergo other co- or posttranslational modifications. We have developed LATE (LysN Amino Terminal Enrichment), a method that uses selective chemical derivatization of α-amines to isolate the N-terminal peptides, in order to improve N-terminome identification in conjunction with other enrichment strategies. We applied LATE alongside another N-termin ...[more]

PMID: 37236440

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			Action	DRS
	Cells_HYTANE_Acet.zip	Other
	Cells_HYTANE_DiMet.zip	Other
	Cells_HYTANE_MetP.zip	Other
	Cells_LATE_Acet.zip	Other
	Cells_LATE_DiMet.zip	Other