Proteomics

Dataset Information

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Structure of nucleosome-bound human PBAF complex


ABSTRACT: BAF and PBAF are mammalian SWI/SNF family chromatin remodeling complexes that possess multiple histone/DNA-binding subunits and create nucleosome-depleted/free regions for transcription activation. Despite previous structural studies and recent advance of SWI/SNF family complexes, it remains incompletely understood how PBAF-nucleosome complex is organized. Here we determined structure of 13-subunit human PBAF in complex with acetylated nucleosome in ADP-BeF3-bound state. Four PBAF-specific subunits work together with nine BAF/PBAF-shared subunits to generate PBAF-specific modular organization, distinct from that of BAF at various regions. PBAF-nucleosome structure reveals six histone-binding domains and four DNA-binding domains/modules, the majority of which directly bind histone/DNA. This multivalent nucleosome-binding pattern, not observed in previous studies, suggests that PBAF may integrate comprehensive chromatin information to target genomic loci for function. Our study reveals molecular organization of subunits and histone/DNA-binding domains/modules in PBAF-nucleosome complex and provides structural insights into PBAF-mediated nucleosome association complimentary to the recently reported PBAF-nucleosome structure.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Li Wang  

LAB HEAD: Yanhui Xu

PROVIDER: PXD036708 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
PBAF-NCP-1.raw Raw
PBAF-NCP-12.xlsx Xlsx
PBAF-NCP-2.raw Raw
PBAF-NCP-3.raw Raw
PBAF-NCP-3.xlsx Xlsx
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Publications

Structure of nucleosome-bound human PBAF complex.

Wang Li L   Yu Jiali J   Yu Zishuo Z   Wang Qianmin Q   Li Wanjun W   Ren Yulei Y   Chen Zhenguo Z   He Shuang S   Xu Yanhui Y  

Nature communications 20221210 1


BAF and PBAF are mammalian SWI/SNF family chromatin remodeling complexes that possess multiple histone/DNA-binding subunits and create nucleosome-depleted/free regions for transcription activation. Despite previous structural studies and recent advance of SWI/SNF family complexes, it remains incompletely understood how PBAF-nucleosome complex is organized. Here we determined structure of 13-subunit human PBAF in complex with acetylated nucleosome in ADP-BeF<sub>3</sub>-bound state. Four PBAF-spe  ...[more]

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