A Natural Biological Adhesive from Snail Mucus for Wound Repair
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Stylommatophora Environmental Sample
SUBMITTER: 
tuo deng
LAB HEAD: Tuo Deng
PROVIDER: PXD036781 | Pride | 2022-12-12
REPOSITORIES: Pride
Dataset's files
Similar Datasets
Project description:The discovery of the natural adhesion phenomena and mechanisms has advanced the information and development of a new generation of tissue adhesives in recent decades. In this study, we developed a natural biological adhesive from snail mucus consisting of a positively charged protein network and a polyanionic glycosaminoglycan network. The malleable bulk adhesive matrix could adhere to wet tissue through multiple interactions. The biomaterial exhibited excellent hemostatic and tissue adhesion properties in vitro and in vivo, and was also effective in accelerating the healing of full-thickness skin wounds in both normal and diabetic rats. The natural biomaterial effectively promoted the polarization of macrophages toward the anti-inflammatory M2 phenotype, alleviated inflammation in chronic wounds, and significantly improved epithelial regeneration and angiogenesis. Its abundant heparin-like glycosaminoglycan component was indispensable. These findings provide theoretical and material insights into bio-inspired tissue adhesives and bioengineered scaffold designs.
2022-11-27 | GSE206113 | GEO
Project description:A Natural Biological Adhesive from Snail Mucus for Wound Repair
| PRJNA849232 | ENA
Project description:Cervical mucus is a viscous fluid functioning as a uterine cervix plug. It is formed and produced by cervical glands located in the cervix. During ovulation, cervical mucus starts to become less viscous, which is a good window for non-invasive sampling. Our study focuses on the proteomic characterization of cervical mucus, which may thus act as a non-invasively acquired source of biomarkers for diseases and physiological conditions of the female genital tract. Our study aimed at two aims - the first is to optimize a proteomic workflow of cervical mucus processing. The second aim is to assess differences in the proteomic composition of cervical mucus in natural ovulatory cycles and IVF cycles with controlled ovarian hyperstimulation. The sampling was done in cooperation with women undergoing intrauterine insemination in a natural ovulatory cycle and women undergoing controlled ovarian hyperstimulation for IVF. The optimization of proteomic workflow including an analysis on an Orbitrap mass spectrometer was performed. The results revealed the protein composition and the differences of the cervical mucus between natural and stimulated uterus.
2023-03-10 | PXD037654 | Pride
Project description:Expression profiling of Drosophila melanogaster halo[AJ] snail[Df(2L)TE116GW11] and halo[AJ] sna[V2] homozygous mutant embryos at two timepoints of embryogenesis. Embryos were manually sorted and assayed at cellularisation stage (stage 5 to early stage 6) and at gastrulation stage (late stage 6 to early stage 8). Homozygous mutant embryos were sorted based on their reduced transparency caused by the recessive mutation of halo[AJ]. Stage-matched halo[AJ] embryos served as wildtype control. Total RNA was extracted, amplified and hybridized to Affymetrix GeneChip Drosophila Genome array version 2. Three biological replicates at each condition were generated.
2014-01-10 | E-MTAB-1598 | biostudies-arrayexpress
Project description:A genomic overview of in vivo binding of a transcription factor Snail in the ascidian early gastrula embryo. Fertilized eggs were electroporated with a construct encoding GFP-tagged Snail. After fixation, chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragments were enriched by immunoprecipitation with an polyclonal antibody specific for GFP (Invitrogen). The immunoprecipitated and WCE (Whole Cell Extract) DNA were amplified by LM-PCR for the Chip analysis. Two independent experiments were performed to reduce experimental errors.
2010-05-06 | E-GEOD-17691 | biostudies-arrayexpress
Project description:Proteins were extracted from the perivitelline fluid of the apple snail Pomacea maculata eggs. And then proteins were seperated on SDS-PAGE gel and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC.
2017-09-25 | PXD006718 | Pride
Project description:Snail is a zinc-finger transcription factor best known for its ability to down-regulate E-cadherin. Its established significance in embryology and organogenesis has been expanded to include a role in the tumor progression of a number of human cancers. In addition to E-cadherin, it has more recently been associated with the down-regulation and up-regulation of a number of other genes that affect important malignant phenotypes. After establishing the presence of up-regulated Snail in human non-small cell lung cancer specimens, we used microarrays to detail the global programme of gene expression in non-small cell lung cancer cell lines stably transduced to over-express Snail as compared to vector control cell lines. Non-small cell lung cancer cell lines (H441, H292, H1437) were stably transduced with a retroviral vector to over-express Snail. Elevated Snail and a corresponding down-regulation of E-cadherin was verified in the Snail over-expressing cell lines as compared to vector control cell lines by Western analysis. RNA extraction was performed and samples submitted to the UCLA Clinical Microarray Core for hybridization to Affymetrix arrays.
2010-06-08 | E-GEOD-16194 | biostudies-arrayexpress
Project description:Epithelial-Mesenchymal Transition (EMT) is thought to contribute to cancer metastasis, but its underlying mechanisms are not well understood. To define early steps in this cellular transformation, we analyzed human mammary epithelial cells with tightly regulated expression of Snail-1, a master regulator of EMT. Following Snail-1 induction, epithelial markers were repressed within 6 hours and mesenchymal genes induced at 24 hours. Snail-1 binding to its target promoters was transient (6-48 hours) despite continued protein expression and it was followed by both transient and long-lasting chromatin changes. To generate a potent reversible EMT-inducing stimulus, we created a Snail-1 retroviral expression construct, using a fused estrogen receptor (ER) response element to mediate regulation by exogenous 4-hydroxy-tamoxifen (4-OHT). Since Snail-1 protein stability and nuclear localization are suppressed by GSK3-beta-mediated phosphorylation, we substituted the six targeted amino acids (ER-Snail-1(6SA)), thus conferring constitutive activity to the induced protein (Zhou et al., 2004, Pubmed ID 15448698). Infection of non-transformed, immortalized human mammary epithelial MCF10A cells with ER-Snail-1(6SA), followed by treatment with 4-OHT, triggered morphological and biomarker characteristics of EMT. At 0, 6, 48 and 120 hours after beginning exposure to 4-OHT in ethanol (or, for controls, ethanol only) we ChIPed Snail-1 and 6 histone marks. We perfomed two replicates of each, except we only had one replicate of H3K27Me3 at 6 hours.
2013-12-18 | E-GEOD-52591 | biostudies-arrayexpress
Project description:Two engineered HMLE cell lines representing various degrees of EMT were treated with a drug in development to target and inhibit SNAIL interaction with PTP53, called GN-25 total RNA was isolated and analyzed in an Agilent two-color experiment. Four biological replicates of each condition were directly compared, GN25 treated compared to untreated reference The impact of GN-25 on gene expression was investigated using two engineered HMLE cell lines: one stably overexpressing Kras another stably overexpressing Kras and SNAIL
2013-05-31 | E-GEOD-44397 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
2013-12-18 | E-GEOD-52593 | biostudies-arrayexpress