Dataset Information

Proteomics of cervical mucus in natural and stimulated cycle

ABSTRACT: Cervical mucus is a viscous fluid functioning as a uterine cervix plug. It is formed and produced by cervical glands located in the cervix. During ovulation, cervical mucus starts to become less viscous, which is a good window for non-invasive sampling. Our study focuses on the proteomic characterization of cervical mucus, which may thus act as a non-invasively acquired source of biomarkers for diseases and physiological conditions of the female genital tract. Our study aimed at two aims - the first is to optimize a proteomic workflow of cervical mucus processing. The second aim is to assess differences in the proteomic composition of cervical mucus in natural ovulatory cycles and IVF cycles with controlled ovarian hyperstimulation. The sampling was done in cooperation with women undergoing intrauterine insemination in a natural ovulatory cycle and women undergoing controlled ovarian hyperstimulation for IVF. The optimization of proteomic workflow including an analysis on an Orbitrap mass spectrometer was performed. The results revealed the protein composition and the differences of the cervical mucus between natural and stimulated uterus.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cervical Mucus

DISEASE(S): Infertility

SUBMITTER: Tomáš Oždian

LAB HEAD: Marián Hajdúch

PROVIDER: PXD037654 | Pride | 2023-03-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20220817_CerHlen_02_exploris_1.raw	Raw
	20220817_CerHlen_02_exploris_2.raw	Raw
	20220817_CerHlen_02_exploris_3.raw	Raw
	20220817_CerHlen_05_exploris_1.raw	Raw
	20220817_CerHlen_05_exploris_2.raw	Raw

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Publications

Proteome Mapping of Cervical Mucus and Its Potential as a Source of Biomarkers in Female Tract Disorders.

Oždian Tomáš T Vodička Jan J Dostál Jiří J Holub Dušan D Václavková Jana J Ješeta Michal M Hamerníková Barbora B Kouřilová Pavla P Malchar Ondřej O Dvořák Vladimír V Hejtmánek Pavel P Sobková Kateřina K Ventruba Pavel P Pilka Radovan R Džubák Petr P Hajdúch Marián M

International journal of molecular sciences 20230105 2

Cervical mucus (CM) is a viscous fluid that is produced by the cervical glands and functions as a uterine cervix plug. Its viscosity decreases during ovulation, providing a window for non-invasive sampling. This study focuses on proteomic characterization of CM to evaluate its potential as a non-invasively acquired source of biomarkers and in understanding of molecular (patho)physiology of the female genital tract. The first objective of this work was to optimize experimental workflow for CM pro ...[more]

PMID: 36674559

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