Dataset Information

Infection cycle and phylogeny of a Polinton-like-virus virophage infecting Phaeocystis globosa

ABSTRACT: Virophages are small dsDNA viruses dependent on a nucleocytoplasmic large-DNA virus infection of a cellular host for replication. Putative virophages infecting algal hosts are classified together with Polinton-like viruses, transposable elements widely found in algal genomes, yet the lack of isolated strains raises questions about their existence as independent entities. We isolated and characterized a virophage (PgVV-14T) co-infecting Phaeocystis globosa with the Phaeocystis globosa virus-14T (PgV-14T).

INSTRUMENT(S): Q Exactive Plus, Q Exactive HF

ORGANISM(S): Phaeocystis Globosa Virus Phaeocystis Globosa Phaeocystis Globosa Virus Virophage Phaeocystis Globosa Virus 14t

SUBMITTER: Oded Kleifeld

LAB HEAD: Oded Beja

PROVIDER: PXD036892 | Pride | 2023-02-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2022-07-12-decoys-contam-globosa_united_Jul2022.fasta.fas	Fasta
	Infection_Time_Course_MF_MBR_LFQ_Jul2022.zip	Other
	PGVV__files_List.txt	Txt
	Purified_Virus_MF_MBR_NewDb_Jul2022.zip	Other
	Seq66830_HF.raw	Raw

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Publications

Isolation and infection cycle of a polinton-like virus virophage in an abundant marine alga.

Roitman Sheila S Rozenberg Andrey A Lavy Tali T Brussaard Corina P D CPD Kleifeld Oded O Béjà Oded O

Nature microbiology 20230126 2

Virophages are small double stranded DNA (dsDNA) viruses that can only replicate in a host by co-infecting with another virus. Marine algae are commonly associated with virophage-like elements such as Polinton-like viruses (PLVs) that remain largely uncharacterized. Here we isolated a PLV that co-infects the alga Phaeocystis globosa with the Phaeocystis globosa virus-14T (PgV-14T), a close relative of the "Phaeocystis globosa virus-virophage" genomic sequence. We name this PLV 'Gezel-14T. Gezel ...[more]

PMID: 36702941

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