Dataset Information

Membrane vesicles (MVs) in Alteromonas macleodii

ABSTRACT: In this study we characterized MV production by six different strains of Alteromonas macleodii, a cosmopolitan marine bacterium.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Alteromonas Macleodii

SUBMITTER: ZIhao Zhao

LAB HEAD: Eduard Fadeev

PROVIDER: PXD037008 | Pride | 2023-07-20

REPOSITORIES: Pride

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Characterization of membrane vesicles in Alteromonas macleodii indicates potential roles in their copiotrophic lifestyle.

Fadeev Eduard E Carpaneto Bastos Cécile C Hennenfeind Jennifer H JH Biller Steven J SJ Sher Daniel D Wietz Matthias M Herndl Gerhard J GJ

microLife 20221220

Bacterial membrane vesicles (MVs) are abundant in the oceans, but their potential functional roles remain unclear. In this study we characterized MV production and protein content of six strains of Alteromonas macleodii, a cosmopolitan marine bacterium. Alteromonas macleodii strains varied in their MV production rates, with some releasing up to 30 MVs per cell per generation. Microscopy imaging revealed heterogenous MV morphologies, including some MVs aggregated within larger membr ...[more]

PMID: 37223730

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Project description:Several studies indicate that adult stem cells may improve the recovery from acute tissue injury. It has been suggested that they may contribute to tissue regeneration by the release of paracrine factors promoting proliferation of tissue resident cells. However, the factors involved remain unknown. In the present study we found that microvesicles (MV) derived from human liver stem cells (HLSC) were able to stimulate in vitro proliferation and apoptosis resistance of human and rat hepatocytes. These effects required internalization of MV in the hepatocytes by an alpha4 integrin-dependent mechanism. However, when treated with RNase, MV despites their internalization were unable to induce hepatocyte proliferation and apoptosis resistance, suggesting an RNA dependent effect. Microarray analysis and quantitative RT-PCR demonstrated that MV were shuttling a specific subset of cellular mRNA, such as mRNA associated in the control of transcription, translation, proliferation and apoptosis. When administered in vivo, MV were found to accelerate the morphological and functional recovery of liver in a model of 70% hepatectomy in rats by inducing an hepatocytes proliferation that was abolished by RNase treatment. Using human AGO2 gene, which is shuttled by MV, as a reporter gene, we found the expression of human AGO2 mRNA and protein in the liver of hepatectomized rats treated with MV. This suggest a translation of the MV shuttled mRNA within hepatocytes of treated rats. Conclusion: these results suggest that MV derived from HLSC may activate a proliferative program in remnant hepatocytes after hepatectomy by a horizontal transfer of specific mRNA subsets. MV contained mRNA was submitted to microarray analysis not to define the amount of mRNA but only to define which transcripts were present. Total RNA was prepared from two independent preparation of vesicles. 250, 500 and 1000 ngs from each preparation were transformed in biotin-labeled cRNA. A simple statistical linear model was used to identify transcript signals linearly correlated to the increment of total RNA concentration used to prepare cRNA.

			Action	DRS
	Amac_AD45-_1_.msf	Msf
	Amac_ATCC27126-_1_.msf	Msf
	Amac_BGP6-_1_.msf	Msf
	Amac_BS11.msf	Msf
	Amac_HOT1A3-_1_.msf	Msf

Dataset Information

Membrane vesicles (MVs) in Alteromonas macleodii

Dataset's files

Publications

Characterization of membrane vesicles in <i>Alteromonas macleodii</i> indicates potential roles in their copiotrophic lifestyle.

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