Proteomics

Dataset Information

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Proteome profile of Methicillin-Resistant Staphylococcus aureus Membrane-Derived Vesicles grown in presence of sub-MIC dosage of Vancomycin


ABSTRACT: Methicillin-resistant Staphylococcus aureus (MRSA) has evolved numerous antimicrobial resistance mechanisms and is identified as a serious public health threat by the World Health Organization and U.S. Centers for Disease Control and Prevention. The glycopeptide vancomycin (VAN) remains a cornerstone of therapy for severe MRSA infections despite increasing reports of therapeutic failure in hospitalized patients with bacteremia or pneumonia. Here we examined the effectiveness of MVs-derived methicillin-resistant SA (MRSA) to counteract vancomycin (VAN). We found that supplementation of a typical MIC assay using bacteriologic and tissue-mimicking media with purified MVs attenuated MRSA susceptibility to VAN but no other examined cell-wall targeting antibiotics. In addition, the purified MVs protected MRSA challenged with sub-therapeutic dosage of VAN against the host’s innate immune system. Additionally, the proteome of MV peptides from VAN exposed MRSA was characterized to determine if protein expression was altered.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus Subsp. Aureus Usa300_tch1516

SUBMITTER: Jack-Ansgar Bruun  

LAB HEAD: Mona Johannessen

PROVIDER: PXD024232 | Pride | 2021-11-03

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
180223_0219_Bishnu_A.raw Raw
180223_0219_Bishnu_B.raw Raw
180223_0219_Bishnu_C.raw Raw
180223_0219_Bishnu_D.raw Raw
180223_180219_Bishnu_MVs.msf Msf
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