Proteomics

Dataset Information

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Lactobacillus Membrane Vesicles


ABSTRACT: Bacterial membrane vesicles have been implicated in a broad range of functions in microbial communities from pathogenesis to gene transfer. Though first thought to be a phenomenon associated with Gram-negative bacteria, vesicle production in Staphylococcus aureus, Lactobacillus plantarum, and other Gram-positives has recently been described. Here we characterize MVs from three different Lactobacillus species (L. acidophilus, L. casei, and L. reuteri), determining that the size and protein composition of Lactobacillus-derived MVs have both similarities and differences with those produced by Gram-negative bacteria. Using proteomics, we identified more than 80 protein components from Lactobacillus-derived MVs, including some that were enriched in the vesicles themselves. For each species, vesicular proteins were categorized based on biological pathway and examined for subcellular localization signals in an effort to identify possible sorting mechanisms for MV proteins. Additionally, differences between MVs of other Lactobacillus species and Gram positive bacteria were highlighted. Information in this study will assist in elucidation of the formation and functions of MVs, as well as the development of therapeutic tools for vaccines, diagnosis, and therapeutic delivery.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Lactobacillus Acidophilus Ncfm Lactobacillus Casei Subsp. Casei Atcc 393 Lactobacillus Reuteri Dsm 20016

SUBMITTER: Scott Dean  

LAB HEAD: Scott A. Walper

PROVIDER: PXD011278 | Pride | 2019-11-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20170607_MV_LA_001_1S_baro.mgf Mgf
20170607_MV_LA_001_1S_baro.pride.mgf.gz Mgf
20170607_MV_LA_001_1S_baro.wiff Wiff
20170607_MV_LA_001_1S_baro.wiff.scan Wiff
20170607_MV_LA_002_1S_baro.mgf Mgf
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Publications

Isolation and characterization of Lactobacillus-derived membrane vesicles.

Dean Scott N SN   Leary Dagmar H DH   Sullivan Claretta J CJ   Oh Eunkeu E   Walper Scott A SA  

Scientific reports 20190129 1


Bacterial membrane vesicles have been implicated in a broad range of functions in microbial communities from pathogenesis to gene transfer. Though first thought to be a phenomenon associated with Gram-negative bacteria, vesicle production in Staphylococcus aureus, Lactobacillus plantarum, and other Gram-positives has recently been described. Given that many Lactobacillus species are Generally Regarded as Safe and often employed as probiotics, the engineering of Lactobacillus membrane vesicles pr  ...[more]

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