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Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion of the Ligandable Proteome

ABSTRACT: Here, we profiled sulfur(VI) fluorides (SVI-F) as reactive functionalities that can modify multiple residues including Lys, Tyr, His and Ser, thus offering utility for targeting almost any protein. These studies provided an in-depth understanding of SVI-F functionalities, including hydrolytic stability, protein reactivity and utility in chemoproteomics

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Aini Vuorinen

LAB HEAD: Jacob Bush

PROVIDER: PXD037048 | Pride | 2024-03-18

REPOSITORIES: Pride

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	ELN17365_10_40per_DIA_88min.raw	Raw
	ELN17365_11_40per_DIA_88min.raw	Raw
	ELN17365_12_40per_DIA_88min.raw	Raw
	ELN17365_13_40per_DIA_88min.raw	Raw
	ELN17365_14_40per_DIA_88min.raw	Raw

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Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion of the Ligandable Proteome.

Gilbert Katharine E KE Vuorinen Aini A Aatkar Arron A Pogány Peter P Pettinger Jonathan J Grant Emma K EK Kirkpatrick Joanna M JM Rittinger Katrin K House David D Burley Glenn A GA Bush Jacob T JT

ACS chemical biology 20230117 2

Here, we report a comprehensive profiling of sulfur(VI) fluorides (SVI-Fs) as reactive groups for chemical biology applications. SVI-Fs are reactive functionalities that modify lysine, tyrosine, histidine, and serine sidechains. A panel of SVI-Fs were studied with respect to hydrolytic stability and reactivity with nucleophilic amino acid sidechains. The use of SVI-Fs to covalently modify carbonic anhydrase II (CAII) and a range of kinases was then inv ...[more]

PMID: 36649130

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