Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Cell stage dependent transcriptional response to leukaemic oncogene expression


ABSTRACT: Acute myeloid leukemia (AML) is characterized by a block in myeloid differentiation the stage of which is dependent on the nature of the transforming oncogene and the developmental stage of the oncogenic hit. This is also true for the t(8;21) translocation which gives rise to the RUNX1/ETO fusion protein and initiates the most common form of human AML. To understand the molecular principles governing this differential action, we used the differentiation of mouse embryonic stem cells expressing an inducible RUNX1/ETO protein into blood cells as a traceable model combined with genome-wide analyses of transcription factor binding and gene expression. We found that RUNX1/ETO interferes with both the activating and repressive function of its normal counterpart, RUNX1, at early and late stages of blood cell development. However, the response of the transcriptional network to RUNX1/ETO expression is stage-specific, highlighting the molecular mechanisms determining specific target cell expansion after an oncogenic hit. High throughput sequencing data have been used to study RUNX1/ETO role in hematopoietic system

ORGANISM(S): Mus musculus

SUBMITTER: Salam Assi 

PROVIDER: E-GEOD-64589 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Developmental-stage-dependent transcriptional response to leukaemic oncogene expression.

Regha Kakkad K   Assi Salam A SA   Tsoulaki Olga O   Gilmour Jane J   Lacaud Georges G   Bonifer Constanze C  

Nature communications 20150528


Acute myeloid leukaemia (AML) is characterized by a block in myeloid differentiation the stage of which is dependent on the nature of the transforming oncogene and the developmental stage of the oncogenic hit. This is also true for the t(8;21) translocation that gives rise to the RUNX1-ETO fusion protein and initiates the most common form of human AML. Here we study the differentiation of mouse embryonic stem cells expressing an inducible RUNX1-ETO gene into blood cells as a model, combined with  ...[more]

Similar Datasets

2015-02-25 | E-GEOD-66248 | biostudies-arrayexpress
2022-09-20 | E-MTAB-10069 | biostudies-arrayexpress
2012-03-27 | E-GEOD-29222 | biostudies-arrayexpress
2014-06-12 | E-GEOD-52496 | biostudies-arrayexpress
2015-05-26 | E-GEOD-62847 | biostudies-arrayexpress
2012-04-23 | E-MTAB-1031 | biostudies-arrayexpress
2012-03-26 | E-GEOD-29225 | biostudies-arrayexpress
2012-08-20 | E-MTAB-830 | biostudies-arrayexpress
2015-07-03 | E-GEOD-64862 | biostudies-arrayexpress
2014-06-12 | E-GEOD-52499 | biostudies-arrayexpress