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Ethylene glycol improves electrospray ionization

ABSTRACT: We evaluated a number of compounds as LC additives to modify the electrospray response and found that ethylene glycol was capable of enhancing nESI response by a factor of twofold on average. This result was comparable to DMSO.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Peng Yu

LAB HEAD: Bernhard Kuster

PROVIDER: PXD004919 | Pride | 2017-07-14

REPOSITORIES: Pride

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			Action	DRS
	150509_HeLa_10.RAW	Raw
	150509_HeLa_11.RAW	Raw
	150509_HeLa_12.RAW	Raw
	150509_HeLa_13.RAW	Raw
	150509_HeLa_14.RAW	Raw

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Publications

Ethylene glycol improves electrospray ionization efficiency in bottom-up proteomics.

Yu Peng P Hahne Hannes H Wilhelm Mathias M Kuster Bernhard B

Analytical and bioanalytical chemistry 20161020 4

Liquid chromatography coupled online to nano-electrospray ionization (nESI) tandem mass spectrometry is the analytical workhorse in the field of proteome research. Dimethyl sulfoxide (DMSO) was recently shown to improve nESI efficiency by a factor of three to ten thus improving the sensitivity and coverage of proteomic experiments. However, relatively few investigations into which solvent additives promote nESI response have been performed at a proteomic scale. Here, we systematically evaluated ...[more]

PMID: 27766361

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