Dataset Information

Decreased CSTB, RAGE and Axl receptor is associated with Zika infection in the human placenta

ABSTRACT: Zika virus (ZIKV) compromises the placental integrity infecting the fetus. However, the mechanisms associated with ZIKV penetration into the placenta leading to fetal infection, are unknown. Cystatin B (CSTB), the receptor for advanced glycation end products (RAGE), and tyrosine-protein kinase receptor UFO (AXL) have been implicated in inflammation. The purpose of this work iis work aims to investigate CSTB, RAGE, and AXL receptor expression in ZIKV infected placental tissues at term. The hypothesis is that in ZIKV-infected placenta, there is overexpression of CSTB and increased inflammation affecting the RAGE and AXL receptor expression. Pathological analyses of 22 placentas were performed to determine changes caused by ZIKV infection. Quantitative proteomics, immunofluorescence, and Western Blot analysis were performed to analyze proteins and pathways affected by ZIKV infection in frozen placenta. Pathological The pathological analysis confirmed decreased size of capillaries, hyperplasia of Hofbauer cells, disruption in the trophoblasts layer, and cell agglutination associated to with ZIKV infection of placental tissue. ZIKV infection was mostly localized to the trophoblast layer. There was a significant decrease in the expression of CSTB, RAGE and AXLCSTB, RAGE and AXL expression in ZIKV positive placenta. This downregulation can impair immune responses and facilitate virus penetration into the placenta. Results are consistent with our previous studies and literature of on ZIKV infection by activation ofng Inflammasome and Pyroptosis through caspase 1 upregulation and affecting the cellular structure, tissue remodeling, and cell differentiation by upregulation of tubulin beta and heat shock protein 27.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Placenta

SUBMITTER: Loyda Melendez

LAB HEAD: Loyda Melendez

PROVIDER: PXD037388 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	TMTAnalysis_GabrielBorges_25Apr2022.mzML	Mzml
	TMTAnalysis_GabrielBorges_25Apr2022.mzid.gz	Mzid
	TMT_PoolA_Fraction1_GabrielBorges_21April2022.raw	Raw
	TMT_PoolA_Fraction2_GabrielBorges_21April2022.raw	Raw
	TMT_PoolA_Fraction3_GabrielBorges_21April2022.raw	Raw

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Publications

Decreased CSTB, RAGE, and Axl Receptor Are Associated with Zika Infection in the Human Placenta.

Borges-Vélez Gabriel G Arroyo Juan A JA Cantres-Rosario Yadira M YM Rodriguez de Jesus Ana A Roche-Lima Abiel A Rosado-Philippi Julio J Rosario-Rodríguez Lester J LJ Correa-Rivas María S MS Campos-Rivera Maribel M Meléndez Loyda M LM

Cells 20221116 22

Zika virus (ZIKV) compromises placental integrity, infecting the fetus. However, the mechanisms associated with ZIKV penetration into the placenta leading to fetal infection are unknown. Cystatin B (CSTB), the receptor for advanced glycation end products (RAGE), and tyrosine-protein kinase receptor UFO (AXL) have been implicated in ZIKV infection and inflammation. This work investigates CSTB, RAGE, and AXL receptor expression and activation pathways in ZIKV-infected placental tissues at term. Th ...[more]

PMID: 36429055

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