Proteomics

Dataset Information

0

Integrated glycomic and proteomic signatures of butyrate-stimulated colorectal cancer cell line differentiation

ABSTRACT: It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation. In this study, specific mucin O-glycans were identified that mark butyrate-induced epithelial differentiation of the intestinal cell line CaCo-2, by applying porous graphitized carbon nano-liquid chromatography with electrospray ionization tandem mass spectrometry (PGC-nanoLC-MS/MS). Moreover, a quantitative proteomic approach was used to decipher changes in the cell proteome. By performing an integrative approach, we generated hypotheses about glycosylation signatures of specific cell adhesion proteins.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Katarina Madunic  

LAB HEAD: Manfred Wuhrer

PROVIDER: PXD037450 | Pride | 2023-05-10

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
E20202002701a.raw Raw
E20202002701b.raw Raw
E20202002702a.raw Raw
E20202002702b.raw Raw
E20202002703a.raw Raw
Items per page:
1 - 5 of 40
altmetric image

Publications

O-Glycomic and Proteomic Signatures of Spontaneous and Butyrate-Stimulated Colorectal Cancer Cell Line Differentiation.

Madunić K K   Luijkx Y M C A YMCA   Mayboroda O A OA   Janssen G M C GMC   van Veelen P A PA   Strijbis K K   Wennekes T T   Lageveen-Kammeijer G S M GSM   Wuhrer M M  

Molecular & cellular proteomics : MCP 20230118 3

Gut microbiota of the gastrointestinal tract provide health benefits to the human host via bacterial metabolites. Bacterial butyrate has beneficial effects on intestinal homeostasis and is the preferred energy source of intestinal epithelial cells, capable of inducing differentiation. It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation. In this study, specific mucin O-glycans were identified that mark butyrate  ...[more]

Similar Datasets

Proteomics Treg cells from human blood differentiate into non-lymphoid tissue-resident effector cells upon TNFR2 costimulation
2024-01-24 | PXD048248 | Pride
Proteomics AMPK activation induces RALDH+ tolerogenic dendritic cells by rewiring glucose and lipid metabolism
2024-07-16 | PXD053979 | Pride
Proteomics Characterization of the Clostridioides difficile 630Δerm putative Pro-Pro endopeptidase CD1597
2024-05-27 | PXD052347 | Pride
Proteomics Oxonium ion guided analysis of quantitative proteomics data reveals site-specific O-glycosylation of anterior gradient protein 2 (AGR2)
2022-02-17 | PXD025805 | Pride
Proteomics N-glycoproteins have a major role in MGL binding to colorectal cancer cell lines
2020-08-10 | PXD020344 | Pride
Proteomics Development of inhibitors, probes, and PROTAC provides a complete toolbox to study PARK7 in the living cell
2024-06-16 | PXD047093 | Pride
Proteomics Cellular Validation of Chemically Improved Inhibitor Identifies Monoubiquitination on OTUB2
2023-06-27 | PXD041346 | Pride
Proteomics Targeting IRE1α reprograms the tumor microenvironment and enhances anti-tumor immunity in prostate cancer
2024-08-28 | PXD044287 | Pride
Proteomics POLR2M-mediated paused transcription silences tumor suppressor MIR139 in MLL-AF9 AML
2021-11-11 | PXD022488 | Pride
Transcriptomics Effect of elevated CO2, warming, added rainfall and added nitrogen on Geranium dissectum growing in a natural grassland
2007-06-01 | E-GEOD-6597 | biostudies-arrayexpress