Proteomics

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Integrated glycomic and proteomic signatures of butyrate-stimulated colorectal cancer cell line differentiation

ABSTRACT: It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation. In this study, specific mucin O-glycans were identified that mark butyrate-induced epithelial differentiation of the intestinal cell line CaCo-2, by applying porous graphitized carbon nano-liquid chromatography with electrospray ionization tandem mass spectrometry (PGC-nanoLC-MS/MS). Moreover, a quantitative proteomic approach was used to decipher changes in the cell proteome. By performing an integrative approach, we generated hypotheses about glycosylation signatures of specific cell adhesion proteins.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Katarina Madunic  

LAB HEAD: Manfred Wuhrer

PROVIDER: PXD037450 | Pride | 2023-05-10

REPOSITORIES: Pride

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Publications

O-Glycomic and Proteomic Signatures of Spontaneous and Butyrate-Stimulated Colorectal Cancer Cell Line Differentiation.

Madunić K K   Luijkx Y M C A YMCA   Mayboroda O A OA   Janssen G M C GMC   van Veelen P A PA   Strijbis K K   Wennekes T T   Lageveen-Kammeijer G S M GSM   Wuhrer M M  

Molecular & cellular proteomics : MCP 20230118 3

Gut microbiota of the gastrointestinal tract provide health benefits to the human host via bacterial metabolites. Bacterial butyrate has beneficial effects on intestinal homeostasis and is the preferred energy source of intestinal epithelial cells, capable of inducing differentiation. It was previously observed that changes in the expression of specific proteins as well as protein glycosylation occur with differentiation. In this study, specific mucin O-glycans were identified that mark butyrate  ...[more]

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