Dataset Information

The claudin-like apicomplexan microneme protein is essential for Plasmodium berghei sporozoite infectivity in the mosquito and mammalian hosts

ABSTRACT: Here we characterize the function of a microneme protein expressed both in merozoites and sporozoites, the claudin-like protein CLAMP. We used a conditional genome editing strategy in a rodent malaria model to generate CLAMP-deficient sporozoites. In the absence of CLAMP, sporozoites failed to invade mosquito salivary glands and mammalian hepatocytes, and showed defects in gliding motility and microneme secretion. Our data establish that CLAMP plays an essential role across Plasmodium invasive stages, and might represent a potential target for transmission-blocking antimalarial strategies.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Plasmodium Berghei Anka

DISEASE(S): Malaria

SUBMITTER: Soumia HAMADA

LAB HEAD: Solenne Chardonnet

PROVIDER: PXD037561 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200122_ProteoPB_1DE11_IP-GFP_Slot1-9_1_783.d.zip	Other
	200122_ProteoPB_1DE11_IP-GFP_Slot1-9_1_783_DA5-2-216.mgf	Mgf
	200122_ProteoPB_1DE11_IP-GFP_Slot1-9_1_783_DA5-2-216.xml	Xml
	210430_MAL-IP_1DE02_IP-Clamp_Slot1-47_1_3591.d.zip	Other
	210430_MAL-IP_1DE02_IP-Clamp_Slot1-47_1_3591_DA5-3-236.mgf	Mgf

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Publications

The claudin-like apicomplexan microneme protein is required for gliding motility and infectivity of Plasmodium sporozoites.

Loubens Manon M Marinach Carine C Paquereau Clara-Eva CE Hamada Soumia S Hoareau-Coudert Bénédicte B Akbar David D Franetich Jean-François JF Silvie Olivier O

PLoS pathogens 20230316 3

Invasion of host cells by apicomplexan parasites such as Toxoplasma and Plasmodium spp requires the sequential secretion of the parasite apical organelles, the micronemes and the rhoptries. The claudin-like apicomplexan microneme protein (CLAMP) is a conserved protein that plays an essential role during invasion by Toxoplasma gondii tachyzoites and in Plasmodium falciparum asexual blood stages. CLAMP is also expressed in Plasmodium sporozoites, the mosquito-transmitted forms of the malaria paras ...[more]

PMID: 36928686

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