Dataset Information

High-throughput Quantitative Glycomics Enabled by 12-plex Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags

ABSTRACT: Glycans, which are widely distributed on most proteins and cell surfaces, are a class of important biomolecules playing important roles in various biological processes such as immune response and cellular communication. Modern mass spectrometry (MS) and novel chemical probes together greatly facilitate the routine analysis of glycans. However, the requirement of high-throughput analysis still calls for advanced tools to be developed. Recently, we devised isobaric multiplex reagents for carbonyl-containing compound (SUGAR) tags for N-glycans analysis capable of processing four samples at a time. To further improve the throughput, we utilized the subtle mass differences among different isotopologues combinations and expanded the multiplexing capacity to twelve channels, a three-fold throughput improvement compared to the original setup and achieved ultra-high throughput N-glycans analysis in a single LC-MS/MS injection. We then applied 12-plex SUGAR tags to profile the N-glycans in four subtypes of human Immunoglobulin G (IgG) and to investigate the N-glycans changes in the endometrial cancer cells (ECC1) treated with Atovaquone, a quinone antimicrobial medication, and a dihydroorotate dehydrogenase (DHODH) inhibitor.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Min Ma

LAB HEAD: lingjun Li

PROVIDER: PXD038501 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	09262022_12plex_SUGAR_Igg_T1.raw	Raw
	12-Plex_SUGAR_SI.docx	Other
	20171115_signal_enhance_rep1.RAW	Raw
	20171115_signal_enhance_rep2.RAW	Raw
	20171115_signal_enhance_rep3.RAW	Raw

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Publications

High-Throughput Quantitative Glycomics Enabled by 12-plex Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags.

Li Miyang M Feng Yu Y Ma Min M Kapur Arvinder A Patankar Manish M Li Lingjun L

Journal of proteome research 20230126 5

Glycans, which are widely distributed on most proteins and cell surfaces, are a class of important biomolecules playing crucial roles in various biological processes such as immune response and cellular communication. Modern mass spectrometry (MS) coupled with novel chemical probes greatly facilitates routine analysis of glycans. However, the requirement of high-throughput analysis still calls for advanced tools to be developed. Recently, we devised isobaric multiplex reagen ...[more]

PMID: 36700627

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