Method development for simplified High-Throughput Plasma Proteomics

ABSTRACT: We developed a simplified proteomics sample preparation method that increased the sensitivity and reproducibility of large-scale plasma samples. Peptide loss was reduced while sensitivity was increased by eliminating the peptide clean-up step and using disposable trap column tips. The simplified High-Throughput Plasma Proteomics (sHTPP) method was evaluated using a systemic approach. We identified and quantified more than 500 proteins in non-depleted plasma samples without missing values as the DIA approach improved in terms of identification and quantification. Furthermore, 96-well plate sample handling with a multi-channel pipet allows for high-throughput sample analysis. In a large-scale clinical trial with 300 plasma samples, we used the method to demonstrate robust and accurate quantifications.

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte

DISEASE(S): Wounds And Injuries

SUBMITTER: Jongmin Woo  

LAB HEAD: Qibin Zhang

PROVIDER: PXD038669 | Pride | 2023-04-03

REPOSITORIES: Pride