Dataset Information

Triggering the polyurethane and polyethylene-degrading potential of filamentous fungi secretomes

ABSTRACT: In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based PU and LDPE, using Impranil and a mixture of long-chain alkanes, not only as sole carbon sources but also as enzyme indicators for polymer degradation. The agar plate screening revealed 3 fungal strains belonging to Fusarium and Aspergillus genera, which were further cultured in presence of the same carbon sources, and their secretome was utilized for polymer degradation. Especially for ether-based PU, the secreted proteins of a Fusarium species reduced the weight and the molecular weight of the sample by 24.5 and 20.4%, respectively, while at the same time the secretome of an Aspergillus species caused changes in the molecular structure of LDPE. The proteomic analysis followed proved that the enzymes induced in presence of Impranil can selectively degrade the urethane bond of ether-based PU, a discovery that offers a new alternative in PU waste treatment. Meanwhile, the mechanism of LDPE degradation was not completely understood, although the presence of oxidative enzymes probably accompanied by reactive oxygen species, could be the main factors contributing to polymer functionalization.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Fusarium Oxysporum Aspergillus Transmontanensis

SUBMITTER: Romanos Siaperas

LAB HEAD: Evangelos Topakas

PROVIDER: PXD038835 | Pride | 2023-04-27

REPOSITORIES: Pride

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Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes.

Taxeidis George G Nikolaivits Efstratios E Siaperas Romanos R Gkountela Christina C Vouyiouka Stamatina S Pantelic Brana B Nikodinovic-Runic Jasmina J Topakas Evangelos E

Environmental pollution (Barking, Essex : 1987) 20230320

The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based pol ...[more]

PMID: 36940913

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Project description:Fusarium fujikuroi is a biotechnologically important fungus due to its almost unique ability to produce gibberellic acids (GAs), a family of phytohormones. The fungus was described about 100 years ago as the causative agent of Bakanae (M-bM-^@M-^\foolish seedlingM-bM-^@M-^]) disease of rice. Apart from GAs, the fungus is known to produce pigments and mycotoxins, but the biosynthetic genes are known for only eight products. Here we present a high-quality genome sequence of the first member of the Gibberella fujikuroi species complex (GFC) that allowed de novo genome assembly with 12 scaffolds corresponding to the 12 chromosomes. In this work we focused on identification of all potential secondary metabolism-related gene clusters and their regulation in response to nitrogen availability by transcriptome, proteome, HPLC-FTMS and ChIP-seq analyses. We show that most of the cluster genes are regulated in a nitrogen-dependent manner, and that expression profiles fit to proteome and ChIP-seq data for some but not all clusters. Comparison with genomes of all available Fusarium species, including the recently sequenced F. mangiferae and F. circinatum, showed only a small number of common gene clusters and provides new insights into the divergence of secondary metabolism in the genus Fusarium. Phylogenetic analyses suggest that some gene clusters were acquired by horizontal gene transfer, while others were present in ancient Fusarim species and have evolved differently by gene duplications and losses. One polyketide synthase (PKS) and one non-ribosomal peptide synthetase (NRPS) gene cluster are unique for F. fujikuroi. Their products were identified by combining overexpression of cluster genes with HPLC-FTMS-based analyses. In planta expression studies suggest a specific role of the PKS19 product in rice infection. Our results indicate that comparative genomics together with the used genome-wide experimental approaches is a powerful tool to uncover new secondary metabolites and to understand their regulation at the transcriptional, translational and epigenetic levels. Examination of 3 different histone modifications, with 2 growth conditions for one of the modifications (Total of 4 samples)

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