Proteomics

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Multiplexed quantitative proteomics of CD4 T cell-mediated postsynaptic dendritic cells (DC)

ABSTRACT: We aimed to characterize the proteome remodeling in DCs induced upon antigen presentation. To do so, we used an in vitro antigen presentation model to generate postsynaptic (psDC) or nonsynaptic (nsDC) DCs similarly to previously reported (Alcaraz-Serna et al., 2021). Bone marrow-derived DCs (BMDCs), which had been activated with LPS and pulsed (psDC) or not (nsDC) with the ovalbumin peptide OVA323-339, were co-cultured with resting CD4+ T cells from OT-II mice to allow cognate antigen-dependent immune synapse formation (psDC) or not (nsDC). DCs were then purified for proteomic analysis by using mass spectrometry based on multiplexed isotopic labeling peptides approach.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell

SUBMITTER: Inmaculada Jorge  

LAB HEAD: Jesús Vázquez Cobos

PROVIDER: PXD039035 | Pride | 2024-01-26

REPOSITORIES: Pride

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IJ_FSM_TMT_1.msf Msf
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IJ_FSM_TMT_2.msf Msf
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Publications

Immune synapse formation promotes lipid peroxidation and MHC-I upregulation in licensed dendritic cells for efficient priming of CD8<sup>+</sup> T cells.

Calzada-Fraile Diego D   Iborra Salvador S   Ramírez-Huesca Marta M   Jorge Inmaculada I   Dotta Enrico E   Hernández-García Elena E   Martín-Cófreces Noa N   Nistal-Villán Estanislao E   Veiga Esteban E   Vázquez Jesús J   Pasqual Giulia G   Sánchez-Madrid Francisco F  

Nature communications 20231025 1

Antigen cognate dendritic cell (DC)-T cell synaptic interactions drive activation of T cells and instruct DCs. Upon receiving CD4<sup>+</sup> T cell help, post-synaptic DCs (psDCs) are licensed to generate CD8<sup>+</sup> T cell responses. However, the cellular and molecular mechanisms that enable psDCs licensing remain unclear. Here, we describe that antigen presentation induces an upregulation of MHC-I protein molecules and increased lipid peroxidation on psDCs in vitro and in vivo. We also sh  ...[more]

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