Proteomics

Dataset Information

0

Multiplexed quantitative proteomics of CD4 T cell-mediated postsynaptic dendritic cells (DC)

ABSTRACT: We aimed to characterize the proteome remodeling in DCs induced upon antigen presentation. To do so, we used an in vitro antigen presentation model to generate postsynaptic (psDC) or nonsynaptic (nsDC) DCs similarly to previously reported (Alcaraz-Serna et al., 2021). Bone marrow-derived DCs (BMDCs), which had been activated with LPS and pulsed (psDC) or not (nsDC) with the ovalbumin peptide OVA323-339, were co-cultured with resting CD4+ T cells from OT-II mice to allow cognate antigen-dependent immune synapse formation (psDC) or not (nsDC). DCs were then purified for proteomic analysis by using mass spectrometry based on multiplexed isotopic labeling peptides approach.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Dendritic Cell

SUBMITTER: Inmaculada Jorge  

LAB HEAD: Jesús Vázquez Cobos

PROVIDER: PXD039035 | Pride | 2024-01-26

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
IJ_FSM_TMT_1.msf Msf
IJ_FSM_TMT_1.raw Raw
IJ_FSM_TMT_2.msf Msf
IJ_FSM_TMT_2.raw Raw
IJ_FSM_TMT_3.msf Msf
Items per page:
1 - 5 of 7
altmetric image

Publications

Immune synapse formation promotes lipid peroxidation and MHC-I upregulation in licensed dendritic cells for efficient priming of CD8<sup>+</sup> T cells.

Calzada-Fraile Diego D   Iborra Salvador S   Ramírez-Huesca Marta M   Jorge Inmaculada I   Dotta Enrico E   Hernández-García Elena E   Martín-Cófreces Noa N   Nistal-Villán Estanislao E   Veiga Esteban E   Vázquez Jesús J   Pasqual Giulia G   Sánchez-Madrid Francisco F  

Nature communications 20231025 1

Antigen cognate dendritic cell (DC)-T cell synaptic interactions drive activation of T cells and instruct DCs. Upon receiving CD4<sup>+</sup> T cell help, post-synaptic DCs (psDCs) are licensed to generate CD8<sup>+</sup> T cell responses. However, the cellular and molecular mechanisms that enable psDCs licensing remain unclear. Here, we describe that antigen presentation induces an upregulation of MHC-I protein molecules and increased lipid peroxidation on psDCs in vitro and in vivo. We also sh  ...[more]

Similar Datasets

Transcriptomics Comparative transcriptome analysis of Th22 and Th17 cells
2013-01-29 | E-GEOD-42332 | biostudies-arrayexpress
Transcriptomics Classical dendritic cells are required for dietary antigen-mediated peripheral regulatory T cell and tolerance induction II
2016-03-28 | E-GEOD-77304 | biostudies-arrayexpress
Transcriptomics Transcription profiling of mouse dendritic subsets in vivo
2008-06-14 | E-GEOD-6259 | biostudies-arrayexpress
Transcriptomics Adenovirus Serotype 5 Vectors Trigger IL-27-Dependent Inhibitory CD4+ T Cell Responses
2016-06-01 | E-GEOD-73118 | biostudies-arrayexpress
Transcriptomics Differential antigen processing by dendritic cell subsets in vivo
2006-12-01 | GSE6259 | GEO
Proteomics Extracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
2022-11-09 | PXD037795 | Pride
Proteomics Biogenesis of HLA ligand presentation in immune cells upon activation reveals changes in peptide length preference
2020-09-02 | PXD020011 | Pride
Transcriptomics Increased antigen cross-presentation but impaired cross-priming after activation of PPARγ
2010-05-08 | E-GEOD-15616 | biostudies-arrayexpress
Transcriptomics scRNA-seq analysis of tumor-infiltrating CD45+ cells from sitagliptin-treated mice
2024-02-21 | GSE228128 | GEO
Transcriptomics Network-based screening identifies sitagliptin as an antitumor drug targeting dendritic cells
2024-02-21 | GSE212712 | GEO