Proteomics

Dataset Information

0

In vivo FAM134B ubiquitination in AMFR KD cells


ABSTRACT: FAM134B is a reticulon-homology domain (RHD)-containing protein that participates in membrane-shaping of the endoplasmic reticulum (ER)8 13. It also functions as a mammalian ER-phagy receptor, mediating the fragmentation and selective degradation of ER sheets in multiple cell types8. However, little is known about the molecular and biophysical mechanisms that control and/or switch between these two FAM134B functions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Adriana Covarrubias-Pinto  

LAB HEAD: Ivan Dikic

PROVIDER: PXD039187 | Pride | 2023-06-19

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
20220826_SFB_EMP_KD_1.raw Raw
20220826_SFB_EMP_KD_2.raw Raw
20220826_SFB_EMP_KD_3.raw Raw
20220826_SFB_EMP_NT_1.raw Raw
20220826_SFB_EMP_NT_2.raw Raw
Items per page:
1 - 5 of 15
altmetric image

Publications


The endoplasmic reticulum (ER) undergoes continuous remodelling via a selective autophagy pathway, known as ER-phagy<sup>1</sup>. ER-phagy receptors have a central role in this process<sup>2</sup>, but the regulatory mechanism remains largely unknown. Here we report that ubiquitination of the ER-phagy receptor FAM134B within its reticulon homology domain (RHD) promotes receptor clustering and binding to lipidated LC3B, thereby stimulating ER-phagy. Molecular dynamics (MD) simulations showed how  ...[more]

Similar Datasets

2023-06-19 | PXD032741 | Pride
2023-06-19 | PXD032743 | Pride
2023-06-19 | PXD032750 | Pride
2023-06-19 | PXD032740 | Pride
2023-06-19 | PXD032721 | Pride
2023-06-19 | PXD039186 | Pride
2023-06-19 | PXD039188 | Pride
2023-06-19 | PXD032718 | Pride
2023-06-19 | PXD032720 | Pride
2023-06-19 | PXD039184 | Pride