Proteomics

Dataset Information

0

Oxidized methionine melanoma epitopes


ABSTRACT: One of the most promising alternatives to chemotherapy in cancer treatment is immunotherapy, particularly, peptide-based immunotherapy in which synthetic peptides derived from tumor-specific antigens are used to trigger an immune response to specifically target cancer cells. Here using custom nanoLC-MS/MS based workflows we examine the possible presentation of peptide-based oxidative variants in complex with Major Histocompatibility Complex (MHC) class I proteins at the surface of melanoma cells. The deposited data contains the raw files associated with mild acidic elutions (MAE) of MHC class I associated peptides from various melanoma cell lines. These were analysed using Data Independent Acquisition (DIA), employing a custom method for MS/MS fragmentation of the native and Met-oxidative modifications of a tyrosinase derived peptide (YMDGTMSQV denoted as YMD peptide). The data contains also the raw files referring to the nanoLC-MS/MS analysis of HPLC purified fractions of various oxidative derivatives of the YMD peptide but also of MAGE-10 native and oxidized forms.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte, Cell Culture

DISEASE(S): Cutaneous Malignant Melanoma 1,Melanoma

SUBMITTER: Cristian Munteanu  

LAB HEAD: Ștefana M. Petrescu

PROVIDER: PXD039318 | Pride | 2023-07-25

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
190530_stdYMD_HPLC_M2M2M6M6.raw Raw
190613_stdYMD_HPLC_M2.raw Raw
190613_stdYMD_HPLC_M6_run01.raw Raw
190613_stdYMD_HPLC_N.raw Raw
190618_stdYMD_HPLC_M2M6.raw Raw
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Publications

Methionine oxidation selectively enhances T cell reactivity against a melanoma antigen.

Chiriţoiu Gabriela N GN   Munteanu Cristian V A CVA   Şulea Teodor A TA   Spiridon Laurenţiu L   Petrescu Andrei-Jose AJ   Jandus Camilla C   Romero Pedro P   Petrescu Ştefana M ŞM  

iScience 20230625 7


The impact of the peptide amino acids side-chain modifications on the immunological recognition has been scarcely explored. We investigate here the effect of methionine oxidation on the antigenicity of the melanoma immunodominant peptide 369-YMDGTMSQV-377 (YMD). Using CD8<sup>+</sup> T cell activation assays, we found that the antigenicity of the sulfoxide form is higher when compared to the YMD peptide. This is consistent with free energy computations performed on HLA-A∗02:01/YMD/TCR complex sh  ...[more]

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