Dataset Information

Genetically divergent Francisella philomiragia associated with septic arthritis, Montevideo, Uruguay

ABSTRACT: Identification of proteins by nanoLC-MS/MS from a colony of Francisella philomiragia isolated from pacient.

INSTRUMENT(S): LTQ

ORGANISM(S): Francisella Philomiragia Fsc154

SUBMITTER: Maria Magdalena Portela

LAB HEAD: Analytical Biochemistry and Proteomics Unit

PROVIDER: PXD039709 | Pride | 2024-01-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	220222_Francisella_philomiragia_uniprot_proteome_UP000001320.fasta	Fasta
	220226_Colony_Francisella_ph.raw	Raw
	220226_Colony_Francisella_ph.txt	Txt
	checksum.txt	Txt

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Genetically divergent <i>Francisella philomiragia</i> associated with septic arthritis, Montevideo, Uruguay.

Riera Nadia N Salazar Cecilia C Rivera Bernardina B Galiana Antonio A Durán Rosario R Portela María Magdalena MM Antelo Virginia V Pi Beatriz B González Óscar Ó Iraola Gregorio G

New microbes and new infections 20231214

Here we report a case of septic arthritis associated with a genetically divergent <i>Francisella philomiragia</i> strain in a patient with chronic rheumatoid arthritis and Adult-onset Still's disease (AOSD) in Maldonado, Uruguay. In this study mass spectrometry together with whole-genome sequencing using Oxford Nanopore technology allowed for the correct identification of the etiologic agent. ...[more]

PMID: 38261949

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Project description:The highly infectious bacterium Francisella tularensis is a facultative intracellular pathogen, whose virulence requires proliferation inside host cells, including macrophages. Although some Francisella determinants of intracellular growth have been identified, much remains to be understood about the pathogenesis of this organism. In particular, how Francisella responds to its intracellular environment could provide clues about its intracellular biology and reveal pathogenic determinants based on their intracellular expression profiles. Here we have performed a global transcriptional profiling of the highly virulent F. tularensis subsp. tularensis Schu S4 strain during its intracellular cycle within primary murine macrophages. Phagocytosed bacteria rapidly responded to their intracellular environment and progressively altered their transcriptional profile over time. Differential gene expression profiles were revealed that correlated with specific intracellular locations of the bacteria. Upregulation of general and oxidative stress response genes was a hallmark of the early phagosomal and late endosomal stages, while induction of a subset of transport and metabolic genes characterized the cytosolic replication stages. Expression of the Francisella Pathogenicity Island (FPI) genes, the functions of which are associated with intracellular proliferation, increased during the intracellular cycle. Similarly, 27 chromosomal loci putatively encoding hypothetical, secreted, outer membrane proteins or transcriptional regulators were identified as upregulated during the intracellular cycle. In-frame deletion of FTT0383, the Schu S4 ortholog of fevR, of FTT0369c or FTT1676 abolished the ability of Schu S4 to either survive or proliferate intracellularly, demonstrating that bacterial factors of intracellular pathogenesis can be identified based on their intracellular expression profile. In conclusion, establishing the intracellular transcriptome of Francisella has revealed important aspects of its intracellular biology and identified novel virulence determinants of this pathogen. Keywords: Time series