Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from multiple strains of Francisella

ABSTRACT: Comparison of expression profiles of strains of Francisella to identify virulence factors We used custom microarrays to detail the global gene expression of four strains of Francisella (Schu4, LVS, OR960246, U112) and identified distinct classes of differentially expressed genes during this process.

ORGANISM(S): Francisella tularensis subsp. holarctica OR96246 Francisella tularensis subsp. tularensis SCHU S4 Francisella Francisella tularensis subsp. novicida U112 Francisella tularensis subsp. holarctica LVS

PROVIDER: GSE68478 | GEO | 2015/05/02

SECONDARY ACCESSION(S): PRJNA282836

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from multiple strains of Francisella

Project description:Comparison of expression profiles of strains of Francisella to identify virulence factors We used custom microarrays to detail the global gene expression of four strains of Francisella (Schu4, LVS, OR960246, U112) and identified distinct classes of differentially expressed genes during this process. All the strains of Francisella were grown to optical density of 0.6 in a chemical defined medium and their expression profiles were compared.

2015-05-02 | E-GEOD-68478 | biostudies-arrayexpress

Novel Small RNA Modulates Virulence in Francisella tularensis

Project description:Most sRNAs control the expression of target genes by interacting with their mRNA. The fvr1 (Francisella virulence RNA 1) gene is found in the IGR between FTL_0777 and FTL_0778 and its sequence does not overlap those of the flanking genes, indicating that its target(s) is located in trans. Base-pairing between a sRNA and target mRNA generally leads to changed translation and often the stability of the mRNA is affected as well. Therefore, to identify possible targets of Fvr1, we compared the transcriptomes of the LVS?fvr1(LVS, for Live Vaccine Strain) and LVS/pfvr1+ strains after growth in liquid broth.

2012-01-31 | E-MEXP-3424 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on solid medium

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown on chocolate agar plates.

2009-05-01 | E-MEXP-1884 | biostudies-arrayexpress

Next Generation Sequencing (NGS) for quantitative transcriptomic analysis of Francisella strains (Francisella novicida, ribD-deficient Francisella novicida , Francisella tularensis)

Project description:To understand differences of gene expression profiles between Francisella strains RNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000. qRT–PCR validation was performed using SYBR Green assays. Our study represents the first detailed differential transcriptomic analysis of Francisella strains , with biologic replicates, generated by RNA-seq technology.

2022-05-31 | GSE174793 | GEO

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on complex liquid medium

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in complex liquid broth.

2009-05-01 | E-MEXP-1891 | biostudies-arrayexpress

Transcription profiling of Francisella tularensis LVS wild type and hfq mutant strains grown on defined liquid medium (Chamberlains medium)

Project description:Comparison of Francisella tularensis LVS wild-type and hfq mutant strains grown in defined liquid medium (Chamberlain's medium)

2009-05-01 | E-MEXP-1892 | biostudies-arrayexpress

An AraC/XylS Family Transcriptional Regulator Modulates the Oxidative Stress Response of Francisella tularensis

Project description:Francisella tularensis is a Gram-negative bacterium that causes a fatal human disease known as tularemia. The Centers for Disease Control have classified F. tularensis as Category A Tier-1 Select Agent. The virulence mechanisms of Francisella are not entirely understood. Francisella possesses very few transcription regulators, and most of these regulate the expression of genes involved in intracellular survival and virulence. The F. tularensis genome sequence analysis reveals an AraC (FTL_0689) transcriptional regulator homologous to the AraC/XylS family of transcriptional regulators. In Gram-negative bacteria, AraC activates genes required for L-arabinose utilization and catabolism. The role of the FTL_0689 regulator in F. tularensis is not known. In this study, we characterized the role of FTL_0689 in gene regulation of F. tularensis and investigated its contribution to intracellular survival and virulence. The results demonstrate that FTL_0689 in Francisella is not required for L-arabinose utilization. Instead, FTL_0689 specifically regulates the expression of the oxidative and global stress response, virulence, metabolism, and other key pathways genes required by Francisella when exposed to oxidative stress. The FTL_0689 mutant is attenuated for intramacrophage growth, and mice infected with the FTL_0689 mutant survive better than wild-type F. tularensis LVS infected mice. Based on the deletion mutant phenotype, FTL_0689 was termed osrR (oxidative stress response regulator). Altogether, this study elucidates the role of the osrR transcriptional regulator in tularemia pathogenesis.

2021-10-13 | GSE183001 | GEO

Microarray analysis of human monocytes infected with Francisella tularensis

Project description:Differential expression in human peripheral blood monocytes between F. novicida-infected and uninfected, and between Francisella tularensis tularensis isolate Schu S4 and uninfected. The goal was to examine genomewide transcriptional reponses to these two strains, and identify differentially-regulated genes that may help explain the virulence of Schu S4. Keywords: Immune Response, Human Monocytes, Bacteria, Francisella

2008-08-13 | GSE12108 | GEO

Characterization of inner and outer membranes proteins from Francisella tularensis strains LVS and Schu S4 and evaluation of possible vaccine candidates.

Project description:The inner and outer membranes were isolated from three biological replicates of two different strains of Francisella tularensis (LVS and Schu S4). Peptides were generated by proteolytic digestion with trypsin. Samples were analyzed on a quadrupole time-of-flight TripleTOF 5600 mass spectrometer and the data searched using Protein Pilot. Weighted spectral counting was performed using peptides that were at least 99% confident. Additionally, the membrane preparations were used to inoculate mice against a challenge with either LVS or SchuS4 strains. Immunoreactive bands from the mouse vaccination study were also extracted, digested with trypsin, and the spectral counts calculated.

2016-12-13 | MSV000080393 | MassIVE

Transcription profiling of Francisella tularensis LVS after heat shock

Project description:Effect of temperature upshift at 44°C for 30 minutes on transcription in Francisella tularensis LVS

2009-07-01 | E-MEXP-2037 | biostudies-arrayexpress