Dataset Information

Comparative Wnt dependent Hydra ECM proteome

ABSTRACT: Comparative proteome of extracted Hydra vulgaris AEP extracellular matrix form Wnt9/10 knock out and Azakenpaullone treated animals. Used to analyse the changes in molecular composition of the mesoglea (ECM) of normal and Wnt depleted animals

INSTRUMENT(S): Q Exactive

ORGANISM(S): Hydra Vulgaris

TISSUE(S): Connective Tissue

SUBMITTER: Frank Stein

LAB HEAD: Suat Oezbek

PROVIDER: PXD039846 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Bert_210716_P1746_MR_SK_Hydra_TP_TMT12_fr1.raw	Raw
	Bert_210716_P1746_MR_SK_Hydra_TP_TMT12_fr10.raw	Raw
	Bert_210716_P1746_MR_SK_Hydra_TP_TMT12_fr11.raw	Raw
	Bert_210716_P1746_MR_SK_Hydra_TP_TMT12_fr12.raw	Raw
	Bert_210716_P1746_MR_SK_Hydra_TP_TMT12_fr2.raw	Raw

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Publications

Wnt/β-catenin signaling induces axial elasticity patterns of Hydra extracellular matrix.

Veschgini Mariam M Suzuki Ryo R Kling Svenja S Petersen Hendrik O HO Bergheim Bruno Gideon BG Abuillan Wasim W Linke Philipp P Kaufmann Stefan S Burghammer Manfred M Engel Ulrike U Stein Frank F Özbek Suat S Holstein Thomas W TW Tanaka Motomu M

iScience 20230316 4

The extracellular matrix (ECM) plays crucial roles in animal development and diseases. Here, we report that Wnt/β-catenin signaling induces the ECM remodeling during Hydra axis formation. We determined the micro- and nanoscopic arrangement of fibrillar type I collagen along Hydra's body axis using high-resolution microscopy and X-ray scattering. Elasticity mapping of the ECM ex vivo revealed distinctive elasticity patterns along the body axis. A proteomic analysis of the ECM ...[more]

PMID: 37009232

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Project description:Lymphedema (LD) is characterized by the accumulation of protein-rich interstitial fluid, lipids and a significant inflammatory cell infiltrate in the limb. It causes a significant morbidity and is a common disabling disease affecting more than 150 million people worldwide, however there is no yet curative treatment. In this study, we found that LD tissues from patients exhibit inflamed gene expression profile compared to their normal arm. This was next confirmed by a lipidomic analysis that revealed severe decrease in arachidonic acid-derived lipid mediators generated by the 15-lipoxygenase (15-LO) in lymphedematous arms. Using a mouse model of lymphedema, we reproduced the etiology of the human pathology including the loss of specialized pro-resolving lipid mediators that play essential role in resolution of inflammation. This was associated with a lack of nonlymphoid PPAR-positive regulatory T cells (Treg) recruitment in the injured limb adipose tissue. Importantly, we identified the lymphatic endothelial 15-LO as responsible for the chemoattraction and survival of this Treg subpopulation. These results were confirmed by an aggravation of LD and degradation of the lymphatic network in an original transgenic mouse model in which ALOX15 gene has been selectively deleted in the lymphatic system (ALOX15lecKO). Importantly, this phenotype was rescued by the injection of ALOX15-expressing lentivectors. These results provide evidence that lymphatic 15-LO may represent a novel therapeutic target for LD by serving as a mediator of nonlymphoid Treg cell population invasion into lymphedematous adipose tissue to resolve inflammation. Experimental workflow: To broadly identify gene expression signatures associated to secondary LD, we performed bulk-RNA sequencing on dermolipectomy tissue samples from women who developed LD after breast cancer. Four patient biopsies (normal arm and LD arm in each patient) were studied and the differential expression analysis (DEseq) followed by a protein-coding RNA profiling.

Dataset Information

Comparative Wnt dependent Hydra ECM proteome

Dataset's files

Publications

Wnt/β-catenin signaling induces axial elasticity patterns of <i>Hydra</i> extracellular matrix.

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