Secretomics of sponge whole-body collapse
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ABSTRACT: Sponges (Porifera) are early-branching Metazoa who do not posess muscles or neurons, however are able to undergo a whole-body movement that involves the closure of their canal system and collapse of an epithelial tent. In this study we profile proteomic responses of the freshwater sponge Spongilla lacustris during agitation induced movements to elucidate the early evolution of coordination. Results from Thermal Proteome Profiling (TPP) experiments of agitated sponges suggest active secretion during the movement. Here, we use tandem mass tag (TMT) labeling-based quantification of proteins in the medium to systematically measure quantitative differences of secretion before and after agitation induced movement. In total, 146 proteins were detected in the medium, 47 of which were significantly upregulated during the movement.
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Spongilla Lacustris
TISSUE(S): Whole Body
SUBMITTER: Frank Stein
LAB HEAD: Detlev Arendt
PROVIDER: PXD044175 | Pride | 2024-01-04
REPOSITORIES: pride
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