Proteomics

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Interactors of trapped protein from mitochondria from HeLa cells with stalled import TMT-MS

ABSTRACT: This study explores the consequences of import failure in mammalian cells; wherein, blocking the import machinery has profound effects on mitochondrial ultra-structure and dynamics, but, surprisingly, does not impact import. The explanation is an astonishing response involving intercellular mitochondrial transfer via tunnelling nanotubes: for the import of healthy mitochondria and jettisoning of those with jammed import sites. These observations support the existence of a widespread mechanism for the rescue of mitochondrial protein import failure. Through proteomics, we identified proteins that interact with the trapped precursor, and may be involved in TNT signalling and formation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Hope Needs  

LAB HEAD: Ian Collinson

PROVIDER: PXD040098 | Pride | 2024-05-24

REPOSITORIES: pride

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Publications

Rescue of mitochondrial import failure by intercellular organellar transfer.

Needs Hope I HI   Glover Emily E   Pereira Gonçalo C GC   Witt Alina A   Hübner Wolfgang W   Dodding Mark P MP   Henley Jeremy M JM   Collinson Ian I  

Nature communications 20240202 1

Mitochondria are the powerhouses of eukaryotic cells, composed mostly of nuclear-encoded proteins imported from the cytosol. Thus, problems with the import machinery will disrupt their regenerative capacity and the cell's energy supplies - particularly troublesome for energy-demanding cells of nervous tissue and muscle. Unsurprisingly then, import breakdown is implicated in disease. Here, we explore the consequences of import failure in mammalian cells; wherein, blocking the import machinery imp  ...[more]

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