Unknown,Transcriptomics,Genomics,Proteomics

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Looking for mitochondrial associated RNA

ABSTRACT: We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which translation was inhibited or the mRNA binding protein context altered (Delta puf3). We used cell fractionation protocols together with microarray to assess the distribution of mRNAs between free and mitochondrion-bound polysomes. Keywords: Mitochondrial-associated RNA localization in cells GSM239122-GSM239127: mitochondrial associated RNA (three biological replicates each in dye swap) GSM239128-GSM239131: mitochondrial associated RNA in presence of 200µg/ml cycloheximide (two biological replicates each in dye swap) GSM239132-GSM239135: mitochondrial associated RNA in presence of 2.1mM puromycin (two biological replicates each in dye swap) GSM239136-GSM239139: mitochondrial associated RNA in Delta Puf3 strain (two biological replicates each in dye swap)

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Stéphane LE CROM 

PROVIDER: E-GEOD-9393 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Yeast mitochondrial biogenesis: a role for the PUF RNA-binding protein Puf3p in mRNA localization.

Saint-Georges Yann Y   Garcia Mathilde M   Delaveau Thierry T   Jourdren Laurent L   Le Crom Stephane S   Lemoine Sophie S   Tanty Veronique V   Devaux Frederic F   Jacq Claude C  

PloS one 20080604 6

The asymmetric localization of mRNA plays an important role in coordinating posttranscriptional events in eukaryotic cells. We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which the mRNA binding protein context and the translation efficiency were altered. We identified Puf3p, a Pumilio family RNA-binding protein, as the first trans-acting factor controlling the MLR phenomenon. This allowed the characterization of two classes of ge  ...[more]

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