Proteomics

Dataset Information

0

Deciphering the interaction between TopBP1 and GINS using crosslinking mass spectrometry


ABSTRACT: Recombinant TopBP1 and GINS were cross linked using the MS non-cleavable crosslinker BS3. Crosslinked proteins were then subsequently either digested directly using the SP3 strategy or first seperated on SDS-PAGE and then in-gel digested. cross-linked peptides were identified by LC-MS. The information from this experiment was used to refine cryo EM structures.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Farnusch Kaschani  

LAB HEAD: Dr Farnusch Kaschani

PROVIDER: PXD040156 | Pride | 2024-05-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ACE_0704_BT01.raw Raw
ACE_0704_BT02.raw Raw
ACE_0704_BT03.raw Raw
ACE_0704_BT04.raw Raw
ACE_0704_BT05.raw Raw
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Publications


Activation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for replication origin firing, and as such for faithful genetic inheritance. Our biochemical and structural studies demonstrate that the helicase activator GINS interacts with TopBP1 through two separate binding surfaces, the first involving a stretch of highly conserved amino acids in the TopBP1-GINI region, the second a surface on TopBP1-BRCT4. The two surfaces bind to opposite ends of the A domain of the GINS s  ...[more]

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