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DNA mimicking 32mer foldamers interfere with in vitro chromatin assembly in drosophila embryo extract

ABSTRACT: Systematic study of the influence of different concentrations of DNA-mimicking foldamers (32mer) on the chromatin-bound bound proteome using an in vitro chromatin assembly extract

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Axel Imhof

LAB HEAD: Axel Imhof

PROVIDER: PXD040158 | Pride | 2023-09-07

REPOSITORIES: Pride

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			Action	DRS
	AI_DNA_mimicking_search.zip	Other
	Hela_100ng_20210818.raw	Raw
	Hela_100ng_20210819_RTshift.raw	Raw
	Hela_100ng_afterMScal_20210823.raw	Raw
	Hela_100ng_afterair_20210820.raw	Raw

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Publications

DNA mimic foldamers affect chromatin composition and disturb cell cycle progression.

Kleene Vera V Corvaglia Valentina V Chacin Erika E Forne Ignasi I Konrad David B DB Khosravani Pardis P Douat Céline C Kurat Christoph F CF Huc Ivan I Imhof Axel A

Nucleic acids research 20231001 18

The use of synthetic chemicals to selectively interfere with chromatin and the chromatin-bound proteome represents a great opportunity for pharmacological intervention. Recently, synthetic foldamers that mimic the charge surface of double-stranded DNA have been shown to interfere with selected protein-DNA interactions. However, to better understand their pharmacological potential and to improve their specificity and selectivity, the effect of these molecules on complex chromatin needs to be inve ...[more]

PMID: 37650653

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Pulldown with DNA mimicking foldamers in preblastoderm Drosophila embryo extract

Project description:16mer and 32mer biotinylated DNA mimics (“foldamers”) bound to streptavidin beads, AP-MS after 1h incubation in Drosophila preblastoderm embryo extract.

2023-09-07 | PXD040157 | Pride

Drosophila embryo extract assisted in vitro chromatin assembly in presence of foldamer

Project description:Drosophila preblastoderm embryo extract assisted chromatin assembly with or without 16mer DNA mimic. Pull down of chromatin fiber to reveal chromatin binders in the system.

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Interaction network of the Cysteine Repeat Modular protein complex of Toxoplasma gondii

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Divergent evolutionary strategies for selective sex chromosome regulation in Drosophila

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2021-06-23 | PXD023747 | Pride

IRF2BP2 counteracts the ATF7/JDP2 heterodimer to prevent inflammatory overactivation in AML cells

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2024-05-16 | PXD041817 | Pride

The histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA-binding winged helix domainThe histone acetyltransferase KAT6A is recruited to unmethylated CpG islands via a DNA-binding winged helix domain

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2023-01-14 | PXD034833 | Pride

Peptide-mediated inhibition of the transcriptional regulator Elongin BC induces apoptosis in cancer cells

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