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GSE1 is a prominent interactor of HDAC1

ABSTRACT: We analysed the composition of histone acetylase 1 (HDAC1)-associated proteins in different cellular backgrounds using quantitative mass spectrometry (MS). First, we affinity purified (AP) C-terminally FLAG-tagged HDAC1 from the nearly haploid human cell line HAP1 (Andersson et al, 1987). To examine cross-species conservation in the composition of HDAC1 complexes we additionally analysed HDAC1-FLAG purifications from mouse embryonic fibroblasts (MEFs). Genetic suppressor element 1 (GSE1) was identified as a prominent interaction partner of HDAC1. Hence, we purified V5-tagged GSE1 from HAP1 cells and analysed co-precipitated proteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Wolfgang Reiter

LAB HEAD: Markus Hartl

PROVIDER: PXD040307 | Pride | 2023-10-23

REPOSITORIES: Pride

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			Action	DRS
	20200820_QExHFX1_RSLC1_Vcelkova_Seiser_ext_Uni_MFPL_HDAC1_KO_0h_2c3_20p_plusiRT.raw	Raw
	20200824_QExHFX1_RSLC1_Vcelkova_Seiser_ext_Uni_MFPL_HDAC1_KO_0h_1c3_10p_plusiRT_repeat.raw	Raw
	20200827_QExHFX1_RSLC1_Vcelkova_Seiser_ext_Uni_MFPL_HDAC1_WT_0h_1c1_10p_plusiRT_repeat.raw	Raw
	20200828_QExHFX1_RSLC1_Vcelkova_Seiser_ext_Uni_MFPL_HDAC1_KO_0h_3c3_10p_plusiRT.raw	Raw
	20200831_QExHFX1_RSLC1_Vcelkova_Seiser_ext_Uni_MFPL_HDAC1_WT_0h_3c1_10p_plusiRT_repeat.raw	Raw

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