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Drosophila brain O-GlcNAc proteome

ABSTRACT: O-GlcNAcylation is a dynamic post-translational modification that diversifies the proteome with spatiotemporal precision in response to various stimuli. Its dysregulation is associated with many neurological disorders that impair cognitive function, and yet identification of phenotype-relevant protein substrates in specific brain regions remains unfeasible. By combining O-GlcNAc binding activity of Clostridium perfringens OGA (CpOGA) with TurboID proximity labeling, we developed an O-GlcNAcylation profiling tool that translates O-GlcNAc modification into biotin conjugation for tissue-specific substrates enrichment. We mapped the O-GlcNAcylated proteome in different brain regions of Drosophila.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Brain

SUBMITTER: haibin yu

LAB HEAD: haibin yu

PROVIDER: PXD040412 | Pride | 2024-04-10

REPOSITORIES: Pride

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			Action	DRS
	ANTENNAL_LOBE_10.raw	Raw
	ANTENNAL_LOBE_11.raw	Raw
	ANTENNAL_LOBE_12.raw	Raw
	ANTENNAL_LOBE_7.raw	Raw
	ANTENNAL_LOBE_8.raw	Raw

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